Effect of garlic on cardiovascular disorders: a review

Nutrition Journal

Subir K Maulik¹  Sanjay K Banerjee¹ 

[1] Departments of Pharmacology, All India Institute of Medical sciences, New Delhi – 110029, India

关键词: cardiovascular;    clinical trial;    animal experiment;    garlic;   

Others  :  1222666
DOI  :  10.1186/1475-2891-1-4

 received in 2002-10-10, accepted in 2002-11-19,  发布年份 2002

【 摘 要 】

Garlic and its preparations have been widely recognized as agents for prevention and treatment of cardiovascular and other metabolic diseases, atherosclerosis, hyperlipidemia, thrombosis, hypertension and diabetes. Effectiveness of garlic in cardiovascular diseases was more encouraging in experimental studies, which prompted several clinical trials. Though many clinical trials showed a positive effect of garlic on almost all cardiovascular conditions mentioned above, however a number of negative studies have recently cast doubt on the efficary of garlic specially its cholesterol lowering effect of garlic. It is a great challenge for scientists all over the world to make a proper use of garlic and enjoy its maximum beneficial effect as it is the cheapest way to prevent cardiovascular disease. This review has attempted to make a bridge the gap between experimental and clinical study and to discuss the possible mechanisms of such therapeutic actions of garlic.

【 授权许可】

   
2002 Banerjee and Maulik; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

【 参考文献 】

• [1]Lawson LD: Garlic: a review of its medicinal effects and indicated active compounds. In Phytomedicines of Europe. Chemistry and Biological Activity. Series 691. Edited by Lawson LD & Bauer R. American Chemical Society, Washington, DC; 1998:176-209.
• [2]Moyers S: Garlic in Health, History and World Cuisine. Suncoast Press, St. Petersburg, FL 1996, 1-36.
• [3]Woodward PW: Garlic and Friends: The History, Growth and Use of Edible Alliums. Hyland House, Melbourne, Australia 1996, 2-22.
• [4]Rivlin RS: Patient with hyperlipidemia who received garlic supplements. Lipid management. Report from the Lipid Education Council 1998, 3:6-7.
• [5]Borek C: Antioxidant health effect of aged garlic extract. J Nutr 2001, 131:1010S-1015S.
• [6]Ryu K, Ide N, Matsuura H, Itakura Y: N alpha-(1-deoxy-D-fructos-1-yl)-L-arginine, an antioxidant compound identified in aged garlic extract. J Nutr 2001, 131:972S-976S.
• [7]Schwartz CJ, Valente AJ, Sprague EA: A modern view of atherogenesis. Am J Cardiol 1993, 71:9b-14b.
• [8]Jain RC: Onion and garlic an experimental cholesterol atherosclerosis in rabbits. Artery 1975, 1:115-125.
• [9]Jain RC: Effect of garlic on serum lipids, coagulability and fibrinolytic activity of blood. Am J Clin Nutr 1977, 30:1380-1381.
• [10]Bordia A, Verma SK, Vyas AK, Khabya BL, Rathore AS, Bhu N, Bedi HK: Effect of essential oil of onion and garlic on experimental atherosclerosis in rabbits. Atherosclerosis 1977, 26:379-386.
• [11]Chang MLW, Johnson MA: Effect of garlic on carbohydrate metabolism and lipid synthesis in rats. J Nutr 1980, 110:931-936.
• [12]Kamanna VS, Chandrasekhara N: Hypocholesteromic activity of different fractions of garlic. Ind J Medical Res 1984, 79:580-583.
• [13]Mand JK, Gupta PP, Soni GL, Singh R: Effect of garlic on experimental atherosclerosis in rabbits. Ind Heart J 1985, 37:183-188.
• [14]Betz E, Weidler R: Die Wirkung von Knoblauchextrakt auf die atheerogenese bei kaninchen. In In: Die anwendung aktueller methoden in der arteriosklerose. Forschung Edited by Betz E. 1989, 304-311.
• [15]Rajasree CR, Rajmohan T, Agusti KT: Biochemical effects of garlic on lipid metabolism in alcohol fed rats. Ind J Exp Biol 1999, 37:243-247.
• [16]Mathew BC, Daniel RS: Hypolipidemic effect of garlic protein substituted for caseinin diet of rats compared to those of garlic oil. Ind J Exp Biol 1996, 34:337-340.
• [17]Qureshi AA, Din ZZ, Abuirameileh N, Burger WC, Ahmed Y, Elson CE: Suppression of avian hepatic lipid metabolism by solvent extracts of garlic: impact on serum lipids. J Nutr 1983, 113:1746-1755.
• [18]Kamanna VS, Chandrasekhara N: Effect of garlic on serum lipoproteins cholesterol levels in albino rats rendered hypercholesteremic by feeding cholesterol. Lipids 1982, 17:483-488.
• [19]Chi MS: Effect of garlic products on lipid metabolism in cholesterol-fed rats. Proc Soc Exp Biol Med 1982, 171:174-178.
• [20]Chi MS, Koh ET, Stewart TJ: Effect of garlic on lipid metabolism in rats fed cholesterol or lard. J Nutr 1982, 112:241-248.
• [21]Abramovitz D, Gavri S, Harats D, Levkovitz H, Mirelman D, Miron T, Eilat-Adar S, Rabinkov A, Wilchek M, Eldar M, Vered Z: Allicin-induced decrease in formation of fatty streaks (atherosclerosis) in mice fed a cholesterol-rich diet. Coron Artery Dis 1999, 10:515-519.
• [22]Efendy JL, Simmons DL, Campbell GR, Campbell JH: The effect of the aged garlic extract, 'Kyolic', on the development of experimental atherosclerosis. Atherosclerosis 1997, 132:37-42.
• [23]Campbell JH, Efendy JL, Smith NJ, Campbell GR: Molecular basis by which garlic suppresses atherosclerosis. J Nutr 2001, 131:1006S-1009S.
• [24]Lutomski J: Klinische Untersuchungen Zur therapeutischen wirksamkeit von llya Rogiff knoblanchpillen mit Rutin. Z Phytotherapia 1984, 5:938-942.
• [25]Luley C, Lehmann-Leo W, Moller B, Martin T, Schwartzkopff W: Lack of efficacy of dried garlic in patients with hyperlipoproteinemia. Arzneimittelforschung / Drug Res 1986, 36:766-768.
• [26]Ziaei S, Hantoshzadeh S, Rezasoltani P, Lamyian M: The effect of garlic tablet on plasma lipids and platelet aggregation in nulliparous pregnants at high risk of preeclampsia. Eur J Obstet Gynecol Reprod Biol 2001, 99:201-206.
• [27]Gardner CD, Chatterjee LM, Carlson JJ: The effect of a garlic preparation on plasma lipid levels in moderately hypercholesterolemic adults. Atherosclerosis 2001, 154:213-220.
• [28]Rahman K, Billington D: Dietary supplementation with aged garlic extract inhibits ADP-induced platelet aggregation in humans. J Nutr 2000, 130:2662-2665.
• [29]Superko HR, Krauss RM: Garlic powder, effect on plasma lipids, postprandial lipemia, low-density lipoprotein particle size, high-density lipoprotein subclass distribution and lipoprotein(a). J Am Coll Cardiol 2000, 35:321-326.
• [30]Byrne DJ, Neil HA, Vallance DT, Winder AF: A pilot study of garlic consumption shows no significant effect on markers of oxidation or sub-fraction composition of low-density lipoprotein including lipoprotein(a) after allowance for non-compliance and the placebo effect. Clin Chim Acta 1999, 285:21-33.
• [31]McCrindle BW, Helden E, Conner WT: Garlic extract therapy in children with hypercholesterolemia. Arch Pediatr Adolesc Med 1998, 152:1089-1094.
• [32]Berthold HK, Sudhop T: Garlic preparations for prevention of atherosclerosis. Curr Opin Lipidol 1998, 9:565-569.
• [33]Isaacsohn JL, Moser M, Stein EA, Dudley K, Davey JA, Liskov E, Black HR: Garlic powder and plasma lipids and lipoproteins: a multicenter, randomized, placebo-controlled trial. Arch Intern Med 1998, 158:1189-1194.
• [34]Simons LA, Balasubramanian S: Von Konigsmark M, Parfitt A, Simons J, Peters W. On the effects of garlic on plasma lipids and lipoproteins in mild hypercholesterolemia. Atherosclerosis 1995, 113:219-225.
• [35]Silagy C, Neil A: Garlic as a lipid lowering agent-a meta-analysis. J R Coll Physician Lond 1994, 28:39-45.
• [36]Warshafsky S, Kamer RS, Sivak SL: Effect of garlic on total serum cholesterol, A meta-analysis. Ann Intern Med 1993, 119:599-605.
• [37]Neil HA, Silagy CA, Lancaster T, Hodgeman J, Vos K, Moore JW, Jones L, Cahill J, Fowler GH: Garlic powder in the treatment of moderate hyperlipidaemia: a controlled trial and meta-analysis. J R Coll Physicians Lond 1996, 30:329-334.
• [38]Stevinson C, Pittler MH, Ernst E: Garlic for treating hypercholesterolemia. A meta-analysis of randomized clinical trials. Ann Intern Med 2000, 133:420-429.
• [39]Orekhov AN, Grunwald J: Effects of garlic on atherosclerosis. Nutrition 1997, 13:656-663.
• [40]Yu-Yan Yeh, Liu L: Cholesterol lowering effect of garlic extracts and organosulfur compounds: Human and animal studies. J Nutr 2001, 131:989S-993S.
• [41]Munday JS, James KA, Fray LM, Kirkwood SW, Thompson KG: Daily supplementation with aged garlic extract, but not raw garlic, protects low density lipoprotein against in vitro oxidation. Atherosclerosis 1999, 143:399-404.
• [42]Lewin G, Popov I: Antioxidant effects of aqueous garlic extract. 2nd communication: Inhibition of the Cu(2+)-initiated oxidation of low density lipoproteins. Arzneimittelforschung 1994, 44:604-607.
• [43]Lau Benjamin HS: Suppression of LDL oxidation by garlic. J Nutr 2001, 131(3S):958S-988S.
• [44]Gebhardt R, Beck H: Differential inhibitory effects of garlic-derived organosulfur compounds on cholesterol biosynthesis in primary rat hepatocyte culture. Lipids 1996, 31:1269-1276.
• [45]Rodger B, Roberty B, Edward S: Fibrinolytic activity in acute myocardial infarction. Am J Clin Pathol 1972, 57:359-363.
• [46]Bordia A, Arora SK, Kothari LK, Jain KC, Rathore BS, Rathore AS, Dube MK, Bhu N: The protective action of essential oils of onion and garlic in cholesterol-fed rabbits. Atherosclerosis 1975, 22:103-109.
• [47]Sainani GS, Desai DB, Natu MN, Katrodia KM, Valame VP, Sainani PG: Onion, garlic, and experimental atherosclerosis. Jpn Heart J 1979, 20:351-357.
• [48]Mirhadi SA, Singh S, Gupta PP: Effect of garlic supplementation to cholesterol-rich diet on development of atherosclerosis in rabbits. Ind J Exp Biol 1991, 29:162-168.
• [49]Bordia AK, Joshi HK, Sandya YK, Bhu N: Effect of essential oil of garlic on serum fibrinalytic activity in patients with coronary artery disease. Atheroselerosis 1977, 28:155.
• [50]Bordia AK, Sodhya SK, Rathore AS, Bhu N: Essential oil of garlic on blood lipids and fibribolytic activity in patients with coronary artery disease. J Assoc Phys Ind 1978, 26:327-33.
• [51]Bordia AK, Sharma KD, Parmar VK, Varma SK: Protective effect of garlic oil on the changes produced by 3 weeks of fatty diet on serum cholesterol serum triglycerides, fibrimolytic acativity and platelet adhesiveness in man. Ind Heart J 1982, 34:86.
• [52]Chutani SK, Bardia A: The effect of fried versus Raw garlic on fibrinolytic activity in man. Atherosclerosis 1988, 38:417-421.
• [53]Sainani GS, Desai DB, Gorha NH, Natu SM, Pise DV, Sainani PG: Effect of dietary garlic and onion on serum lipid profile in Jain Community. Ind J of Med Res 1979, 69:776-780.
• [54]Arora RC, Arora S: Comparative effects of clofibrate, garlic and onion on alimentary hyperlipemia. Atherosclerosis 1981, 39:447-452.
• [55]Arora RC, Arora S, Gupta RK: The long-term use of garlic in ischemic heart disease. Atherosclerosis 1981, 40:175-179.
• [56]Legnani C, Frascaro M, Guazzaloca G, Ludovici S, Cesarano G, Coccheri S: Effects of a dried garlic preparation on fibrinolysis and platelet aggregation in healthy subjects. Arzneimittelforschung 1993, 43:119-122.
• [57]Bordia A, Verma SK, Srivastava KC: Effect of garlic (Allium sativum) on blood lipids, blood sugar, fibrinogen and fibrinolytic activity in patients with coronary artery disease. Prostaglandins Leukot Essent Fatty Acids 1998, 58:257-263.
• [58]Bordia AK, Joshi HK: Garlic on fibrinolytic activity in cases of acute myocardial infarction. J Assoc Physiol Ind 2978, 26:323-326.
• [59]Harfenist WJ, Murry RK, Murry RK, Mayes PA, Grannen DK, Rodwell VW: Plasma proteins, immunoglobulin and clotting factors. In Harper's Biochemistry. 25th edition. Edited by Barnes DA. McGraw-Hill, New York, A Lange Medical Book; 2000:737-762.
• [60]Ali M, Thomson M, Alnaqeeb MA, al-Hassan JM, Khater SH, Gomes A: Antithrombotic activity of garlic: its inhibition of the synthesis of thromboxane-B2 during infusion of arachidonic acid and collagen in rabbits. Prostaglandins Leukot Essent Fatty Acids 1990, 41:95-99.
• [61]Srivastava KC: Effects of aqueous extracts of onion, garlic and ginger on platelet aggregation and metabolism of arachidonic acid in the blood vascular system: in vitro study. Prostaglandins Leukot Med 1984, 13:227-235.
• [62]Ali M: Mechanism by which garlic (Allium sativum) inhibits cyclooxygenase activity. Effect of raw versus boiled garlic extract on the synthesis of prostanoids. Prostaglandins Leukot Essent Fatty Acids 1995, 53:397-400.
• [63]Ali M, Bordia T, Mustafa T: Effect of raw versus boiled aqueous extract of garlic and onion on platelet aggregation. Prostaglandins Leukot Essent Fatty Acids 1999, 60:43-47.
• [64]Laurence WVD, John DF: Garlic extract prevents acute platelet thrombus formation in stenosed canine coronary arteries. Am Heart J 1989, 117:973-975.
• [65]Thomson M, Mustafa M, Ali M: Thromboxane-B(2) levels in serum of rabbits receiving a single intravenous dose of aqueous extract of garlic and onion. Prostaglandins Leukot Essent Fatty Acids 2000, 63:217-221.
• [66]el-Sabban F, Fahim MA, Radwan GM, Zaghloul SS, Singh S: Garlic preserves patency and delays hyperthermia-induced thrombosis in pial microcirculation. Int J Hyperthermia 1996, 12:513-525.
• [67]el-Sabban F, Radwan GM: Influence of garlic compared to aspirin on induced photothrombosis in mouse pial microvessels, in vivo. Thromb Res 1997, 88:193-203.
• [68]Apitz-Castro R, Escalante J, Vargas R, Jain MK: Ajoene, the antiplatelet principle of garlic, synergistically potentiates the antiaggregatory action of prostacyclin, forskolin, indomethacin and dypiridamole on human platelets. Thromb Res 1986, 42:303-311.
• [69]Apitz-Castro R, Badimon JJ, Badimon L: A garlic derivative, ajoene, inhibits platelet deposition on severely damaged vessel wall in an in vivo porcine experimental model. Thromb Res 1994, 75:243-249.
• [70]Apitz-Castro R, Badimon JJ, Badimon L: Effect of ajoene, the major antiplatelet compound from garlic, on platelet thrombus formation. Thromb Res 1992, 68:145-155.
• [71]Makheja AN, Bailey JM: Antiplatelet constituents of garlic and onion. Agents Actions 1990, 29:360-363.
• [72]Bordia A: Effect of garlic on human platelet aggregation in vitro. Atherosclerosis 1978, 30:355-360.
• [73]Vanderhock JY, Makheja AN, Bailey JM: Inhibition of fatty acid oxygenases by onion and garlic acts. Evidence for the mechanism by which these oils inhibit platelet aggregation. Biochem Pharmacol 1980, 29:3169-3173.
• [74]Apitz-Castro R, Cabrera S, Cruz MR, Ledezma E, Jain MK: Effects of garlic extract and of three pure components isolated from it on human platelet aggregation, arachidonate metabolism, release activity and platelet ultrastructure. Thromb Res 1983, 32:155-169.
• [75]Srivsatava KC: Evidence for the mechanism by which garlic inhibitors platelet aggregation. Prostaglandin Leukot Med 1986, 22:313-321.
• [76]Samson RR: Effects of dietary garlic and temporal drift on platelet aggregation. Atherosclerosis 1982, 44:119-120.
• [77]Harenberg J, Giese C, Zimmermann R: Effect of dried garlic on blood coagulation, fibrinolysis, platelet aggregation and serum cholesterol levels in patients with hyperlipoproteinemia. Atherosclerosis 1988, 74:247-249.
• [78]Kiesewetter H, Jung F, Pindur G, Jung EM, Mrowietz C, Wenzel E: Effect of garlic on thrombocyte aggregation, microcirculation, and other risk factors. Int J Clin Pharmacol Ther Toxicol 1991, 29:151-155.
• [79]Steiner M, Lin RS: Changes in platelet function and susceptibility of lipoproteins to oxidation associated with administration of aged garlic extract. J Cardiovasc Pharmacol 1998, 31:904-908.
• [80]Boullin DJ: Garlic as a platelet inhibitor. Lancet 1981, 1:776-777.
• [81]Wagner H, Wierer M, Fessler B: Effects of garlic constituents on arachidonate metabolism. Planta Med 1987, 53:305-306.
• [82]Srivastava KC, Tyagi OD: Effects of a garlic-derived principle (ajoene) on aggregation and arachidonic acid metabolism in human blood platelets. Prostaglandins Leukot Essent Fatty Acids 1993, 49:587-595.
• [83]Apitz-Castro R, Ledezma E, Escalante J, Jain MK: The molecular basis of the antiplatelet action of ajoene: direct interaction with the fibrinogen receptor. Biochem Biophys Res Commun 1986, 141:145-150.
• [84]Jamaluddin MP, Krishnan LK, Thomas A: Ajoene inhibition of platelet aggregation: possible mediation by a hemoprotein. Biochem Biophys Res Commun 1988, 153:479-486.
• [85]Ariga T, Oshiba S, Tamada T: Platelet aggregation inhibitor in garlic. Lancet 1981, 1:150-151.
• [86]Block E, Ahmad S, Jain MK, Crecely RW, Apitz Castro R, Cruz MR: (E,Z) Ajoene: A potent antithrombic agent from garlic. J Amer Chem Soc 1984, 106:8295-8296.
• [87]Kiesewetter H, Jung F, Jung EM, Mroweitz C, Koscielny J, Wenzel E: Effect of garlic on platelet aggregation in patients with increased risk of juvenile ischaemic attack. Eur J Clin Pharmacol 1993, 45:333-336.
• [88]Morris J, Burke V, Mori TA, Vandongen R, Beilin LJ: Effects of garlic extract on platelet aggregation: a randomized placebo-controlled double-blind study. Clin Exp Pharmacol Physiol 1995, 22:414-417.
• [89]Steiner M, Li W: Aged garlic extract, a modulator of cardiovascular risk factors: a dose-finding study on the effects of AGE on platelet functions. J Nutr 2001, 131(3s):980S-984S.
• [90]Schulz V, Hansel R, Tyler VE: Cardiovascular system. In Rational Phytotherapy; physicians' guide to herbal medicine. Springer-verlag, Berlin; 2001:107-168.
• [91]Sial AY, Ahmed SJ: Study of the hypotensive action gerlic extract in experimental animals. J Pak Med Assoc 1982, 32:237-239.
• [92]Rashid A, Khan HH: The mechanism of hypotensive effect of garlic extract. J Pak Med Assoc 1985, 35:357-362.
• [93]Chanderkar AG, Jain PK: Analysis of hypotensive action of Allium sativum (garlic). Ind J Physiol Pharmacol 1973, 17:132-133.
• [94]Pantoja CV, Chiang Ch L, Norris BC, Concha JB: Diuretic, natriuretic and hypotensive effects produced by Allium sativum (garlic) in anaesthetized dogs. J Ethnopharmacol 1991, 31:325-331.
• [95]Banerjee AK: Effect of aqueous extract of garlic on arterial blood pressure of normotensive and hypertensive rats. Artery 1976, 2:369.
• [96]Ruffin J, Hunter SA: An evaluation of the side effects of garlic as an antihypertensive agent. Cytobias 1983, 37:85-89.
• [97]Foushee DB, Ruffin J, Banerjee U: Garlic as a natural agent for the treatment of hypertension: A preliminary report. Cytobios 1982, 34:145-152.
• [98]Malik ZA, Siddiqui S: Hypotensive effect of freeze dried garlic (Allium sativum) sap in dog. J Pak Med Assoc 1981, 31:12-13.
• [99]Elkayam A, Mirelman D, Peleg E, Wilchek M, Miron T, Rabinkov A, Sadetzki S, Rosenthal T: The effects of allicin and enalapril in fructose-induced hyperinsulinemic hyperlipidemic hypertensive rats. Am J Hypertens 2001, 14:377-381.
• [100]Ali M, Al-Qattan KK, Al-Enezi F, Khanafer RM, Mustafa T: Effect of allicin from garlic powder on serum lipids and blood pressure in rats fed with a high cholesterol diet. Prostaglandins Leukot Essent Fatty Acids 2000, 62:253-259.
• [101]Kaye AD, De Witt BJ, Anwar M, Smith DE, Feng CJ, Kadowitz PJ, Nossaman BD: Analysis of responses of garlic derivatives in the pulmonary vascular bed of the rat. J Appl Physiol 2000, 89:353-358.
• [102]Al-Qattan KK, Khan I, Alnaqeeb MA, Ali M: Thromboxane-B2, prostaglandin-E2 and hypertension in the rat 2-kidney 1-clip model: a possible mechanism of the garlic induced hypotension. Prostaglandins Leukot Essent Fatty Acids 2001, 64:5-10.
• [103]Kim-Park S, Ku DD: Garlic elicits a nitric oxide-dependent relaxation and inhibits hypoxic pulmonary vasoconstriction in rats. Clin Exp Pharmacol Physiol 2000, 27:780-786.
• [104]Fallon MB, Abrams GA, Abdel-Razek TT, Dai J, Chen SJ, Chen YF, Luo B, Oparil S, Ku DD: Garlic prevents hypoxic pulmonary hypertension in rats. Am J Physiol 1998, 275(2 Pt 1):L283-L287.
• [105]Brandle M, al Makdessi S, Weber RK, Dietz K, Jacob R: Prolongation of life span in hypertensive rats by dietary interventions. Effects of garlic and linseed oil. Basic Res Cardiol 1997, 92:223-232.
• [106]Leoper M, DeBray M: Hypotensive effect of tincture of garlic. Prog Med 1921, 36:391-392.
• [107]Damru F: The use of garlic concentrate in vascular hypertension. Med Rec 1941, 153:249-251.
• [108]Piotrowski'ail en GL: therapeutique. Praxis 1948, 26:488-492.
• [109]Konig FK, Scineider B: Knoblauch bessert Durch-blutungstorungen Arztliche Praxis. 1986, 38:44-35.
• [110]Auer W, Eiber A, Hertkom E, Kohrle U, Lenz A, Mader F, Merx W, Otto G, Schmid-Oto B, benheim H: Hypertonie and Hyperlipidamie: In leichterenauch Knoblauch. Der Allgemeinarzi 1989, 3:205-208.
• [111]Petkov V: Plants and hypotensive, antiatheromatous and coronarodilatating action:. Am J Chin Med 1979, 7:197-236.
• [112]Zheziang Institute of Traditional Chinese Medicine: The effect of essential oil of garlic on hyperlipemia and platelet aggregation – an analysis of 308 cases. Cooperative Group for Essential Oil of Garlic. J Tradit Chin Med 1986, 6:117-120.
• [113]Silagy CA, Neil HA: A meta-analysis of the effect of garlic on blood pressure. J Hyperten 1994, 12:463-468.
• [114]Sendl A, Elbl G, Steinke B, Redl K, Breu W, Wagner H: Comparative pharmacological investigations of Allium ursinum and Allium sativum. Planta Medica 1992, 58:1-7.
• [115]Qidwai W, Qureshi R, Hasan SN, Azam SI: Effect of dietary garlic (Allium Sativum) on the blood pressure in humans – a pilot study. J Pak Med Assoc 2000, 50(6):204-207.
• [116]Steiner M, Khan AH, Holbert D, Lin RI: A double-blind crossover study in moderately hypercholesterolemic men that compared the effect of aged garlic extract and placebo administration on blood lipids. Am J Clin Nutr 1996, 64:866-870.
• [117]Jain AK, Vargas R, Gotzkowsky S, McMahon FG: Can garlic reduce levels of serum lipids? A controlled clinical study. Am J Med 1993, 94:632-635.
• [118]McMahon FG, Vargas R: Can garlic lower blood pressure? A pilot study. Pharmacotherapy 1993, 13:406-407.
• [119]Auer W, Eiber A, Hertkom E, Kohrle U, Lenz A, Mader F, Merx W, Otto G, Schmid-Oto B, benheim H: Hypertonie and Hyperlipidamie: In leichterenauch Knoblauch. Der Allgemeinarzi 1989, 3:205-208.
• [120]Zimmermann W, Zimmermann B: Reduction in elevated blood lipids in hospitalised patients by a standardised garlic preparation. Br J Clin Prac 1990, 44(suppl 69):20-23.
• [121]Vorberg G, Schneider B: Therapy with garlic: results of a placebo-controlled, double-blind study. Br J Clin Prac 1990, 44(suppl 69):7-11.
• [122]Granner DK, Murry RK, Mayes PA, Grannen DK, Rodwell VW: Hormones of the pancreas and gastrontestinal tract. In Harper's Biochemistry. 25th edition. Edited by Barnes DA. McGraw-Hill, New York, A Lange Medical Book; 2000:610-626.
• [123]Ohaeri OC: Effect of garlic oil on the levels of various enzyme in the serum and tissue of streptozotocin diabtic rats. Biosci Rep 2001, 21:19-24.
• [124]Patumraj S, Tewit S, Amatyakul S, Jariyapongskul A, Maneesri S, Kasantikul V, Shepro D: Comparative effects of garlic and aspirin on diabetic cardiovascular complications. Drug Deliv 2000, 7:91-96.
• [125]Swanston-Flatt SK, Day C, Bailey CJ, Flatt PR: Traditional plant treatments for diabetes. Studies in normal and streptozotocin diabetic mice. Diabetologia 1990, 33:462-464.
• [126]Farva D, Goji LA, Joseph PK, Augusti KT: Effects of garlic oil on streptozotocin-diabetic rats maintained on normal and high fat diets. Indian J Biochem Biophys 1986, 23:24-27.
• [127]Kumar GR, Reddy KP: Reduced nociceptive responses in mice with alloxan induced hyperglycemia after garlic (Allium sastivum) teratment. Indian J Exp Biol 1999, 37:662-666.
• [128]Augusti KT, Sheela CG: Antiperoxide effect of S-allyl cysteine sulfoxide, a insulin secretagogue, in diabetic rats. Experientia 1996, 52:115-120.
• [129]Sheela CG, Kumud K, Augusti KT: Anti-diabetic effect of onion and garlic sulfoxide amino acids in rats. Planta Medica 1995, 61:356-357.
• [130]Sheela CG, Augusti KT: Antidiabetic effects of S-allyl cysteine sulphoxide isolated from garlic Allium sativum Linn. Indian J Exp Biol 1992, 30:523-526.
• [131]Jain RC, Vyas CR: Garlic in alloxan-induced diabetic rabbits. Am J Clin Nutr 1975, 28:684-685.
• [132]Mathew PT, Augusti KT: Studies on the effect of allicin (diallyl disulphide-oxide) on alloxan diabetes I. Hypoglycaemic action and enhancement of serum insulin effect and glycogen synthesis. Indian J Biochem Biophys 1973, 10:209-212.
• [133]Kasuga S, Ushijima M, Morihara N, Itakura Y, Nakata Y: Effect of aged garlic extract (AGE) on hyperglycemia induced by immobilization stress in mice. Nippon Yakurigaku Zassh 1999, 114:191-197.
• [134]Jain RC, Vyas CR: Hypoglycemic action of onion and garlic. Lancet 1973, 2:1491.
• [135]Zhang XH, Lowe D, Giles P, Fell S, Connock MJ, Maslin DJ: Gender may affect the action of garlic oil on plasma cholesterol and glucose levels of normal subjects. J Nutr 2001, 131:1471-1478.
• [136]Ali M, Thomson M: Consumption of garlic clove a day could be beneficial in preventing thrombosis. Prostaglandins Leukot Essent Fatty acids 1995, 53:211-212.
• [137]Li G, Shi Z, Jia H, Ju J, Wang X, Xia Z, Qin L, Ge C, Xu Y, Cheng L, Chen P, Yuan G: A clinical investigation on garlicin injectio for treatment of unstable angina pectoris and its actions on plasma endothelin and blood sugar levels. J Tradit Chin Med 2000, 20:243-246.
• [138]Rietz B, Belagyi J, Torok B, Jacob R: The radical scavenging ability of garlic examined in various models. Bolletino Chimico Farmaceutico 1995, 134:69-76.
• [139]Martin N, Bardisa L, Pantojaa C, Vargas M, Quezaada P, Valenzuela J: Antiarrhythmic profile of a gaarlic dialystate assaay ed in dogs and isolaated atrial preparations. J Ethanophrmacol 1994, 43:1-8.
• [140]Martin N, Bardisa L, Pantojaa C, Vargas M, Quezaada P, Valenzuela J: Antiarrhythmic profile of a gaarlic dialystate assaay ed in dogs and isolaated atrial preparations. J Ethanophrmacol 1994, 43:1-8.
• [141]Aqel MB, Gharaibah MN, Salhab AS: Direct relaxant effects of garlic juice on smooth and cardiac muscles. J Ethnopharmacol 1991, 33:13-19.
• [142]Tongia SK: Effects of intravenous garlic Juice Aallium sativum on rat Electrocardiogram. Ind J Physiol Pharmacol 1984, 28:250-252.
• [143]Banerjee SK, Maulik M, Mancahanda SC, Dinda AK, Gupta SK, Maulik SK: Dose-dependent induction of endogenous antioxidants in rat heart by chronic administration of garlic. Life Scicences 2002, 70:1509-1518.
• [144]Banerjee SK, Maulik M, Manchanda SC, Dinda AK, Das TK, Maulik SK: Garlic-induced alteration in rat liver and kidney morphology and associated changes in endogenous antioxidant status. Food Chem Toxicol 2001, 39:793-797.
• [145]Imai J, Ide N, Nagae S, Moriguchi T, Matsuura H, Itakura Y: Antioxidant and radical scavenging effects of aged garlic extract and its constituents. Planta Med 1994, 60:417-420.
• [146]Geng Z, Lau B: Aged garlic extract modulates glutathione redox cycle and superoxide dismutase activity in vascular endothelial cells. Phytother Res 1997, 11:54-56.
• [147]Wei Z, Lau BHA: Garlic inhibits free radical generation and augments antioxidant enzyme activity in vascular endothelial cells. Nutr Res 1998, 18:61-70.
• [148]Banerjee SK, Maulik M, Gupta SK, Manchanda SC, Dinda AK, Maulik SK: Effect of chronic garlic intake on endogenous antioxidants and ischemic-reperfusion injury in isolated rat heart. Ind J Pharmacol 2001, 33:298.
• [149]Mukherjee S, Maulik M, Talwar KK, Dinda AK, Maulik SK: Effect of chronic raw garlic administration in adriamycin induced oxidant stress in rat hearts. Ind J Pharmacol 2001, 33:297.
• [150]Kojima R, Epstein CJ, Mizui T, Carlson E, Chaqn PH: Protective effects of aged garlic extracts on doxorubicin induced cardiotoxicity in the mouse. Nutr Cancer 1994, 22:163-173.
• [151]Ciplea AG, Richter KD: The protective effect of Allium sativum and crataegus on isoprenaline-induced tissue necrosis in rats. Arzneim-Forsch / Drug Res 1988, 38(II):1583-1592.
• [152]Isensee H, Rietz B, Jacob R: Cardioprotective action of garlic (Allium sativum). Arzneim Forsch / Drug Res 1993, 43:94-98.
• [153]Yamasaki T, Lau BH: Garlic compounds protect vascular endothelial cells from oxidant injury. Nippon Yakurigaku Zasshi 1997, 110(Suppl 1):138P-141P.
• [154]Breithaupt-Grogler K, Belz GG: Epidemiology of the arterial stiffness. Pathol Biol (Paris) 1999, 47:604-613.
• [155]Breithaupt-Grogler K, Ling M, Boudoulas H, Belz GG: Protective effect of chronic garlic intake on elastic properties of aorta in the elderly. Circulation 1997, 96:2649-2655.
• [156]Kiesewetter H, Jung F, Mrowietz C, Pindur G, Heiden M, Wenzel E, Gu LD: Effects of garlic on blood fluidity and fibrinolytic activity: a randomised, placebo-controlled, double-blind study. Br J Clin Pract 1990, 69:24-29.
• [157]Jung EM, Jung F, Mrowietz C, Kiesewetter H, Pindur G, Wenzel E: Influence of garlic powder on cutaneous microcirculation. A randomized placebo-controlled double-blind cross-over study in apparently healthy subjects. Arzneimittelforschung 1991, 41:626-630.
• [158]Das T, Roychoudhury A, Sharma A, Talukder G: Effects of crude garlic extract on mouse chromosomes in vivo. Food and Chemical Toxicology 1996, 34:43-47.
• [159]Augusti KT: Therapeutic values of onion (Allium cepa L) and garlic (Allium sativum L). Ind J Exp Biol 1996, 34:634-640.
• [160]Nakagawa S, Masamoto K, Sumiyoshi H, Kunihiro K, Fuwa T: Effect of raw and extracted aged garlic on growth of young rats and their organ after peroral administration. J Toxicol Sci 1980, 5:91-112.
• [161]Joseph PK, Rao KR, Sundaresh CS: Toxic effects of garlic extract and garlic oil in rats. Ind J Exp Biol 1989, 27:977-979.
• [162]Chen L, Hong JY, So E, Hussain AH, Cheng WF: Decrease of hepatic catalase level by treatment with diallyl sulfide and garlic homogenates in rats and mice. J Biochem Mol Toxicol 1999, 13:127-133.
• [163]Augusti KT, Mathew PT: Effect of allicin on certain enzymes of liver after a short term feeding to normal rats. Experentia 1975, 31:148-149.
• [164]Dixit VP, Joshi S: Effects of chronic administration of garlic (Allium sativum Linn) on testicular function. Ind J Exp Biol 1982, 20:534-536.
• [165]Egen-Schwind C, Eckard R, Kemper FH: Metabolism of garlic constituents in the isolated perfused rat liver. Planta Medica 1992, 58:301-305.
• [166]Sheen LY, Li CK, Sheu SF, Meng RHC, Tsai SJ: Effect of the active principle of garlic-diallyl sulfide- on cell viability, detoxification capability and the antioxidation system on primary rat hepatocytes. Food Chem Toxicol 1996, 34:971-978.
• [167]Sheen LY, Chen HW, Kung YL, Liu CT, Lii CK: Effects of garlic oil and its organosulfur compounds on the activities of hepatic drug-metabolizing and antioxidant enzymes in rats fed high- and low-fat diets. Nutr Cancer 1999, 35:160-166.
• [168]Gallwitz H, Bonse S, Martinez-Cruz A, Schlichting I, Schumacher K, Krauth-Siege RL: Ajoene is an inhibitor and subversive substrate of human glutathione reductase and Trypanosoma cruzi trypanothione reductase: crystallographic, kinetic, and spectroscopic studies. J Med Chem 1999, 42:364-372.
• [169]Rabinkov A, Miron T, Mirelman D, Wilchek M, Glozman S, Yavin E, Weiner L: S-Allylmercaptoglutathione: the reaction product of allicin with glutathione possesses SH-modifying and antioxidant properties. Biochim Biophys Acta 2000, 1499:144-153.
• [170]Beck E, Grunwald J: Allium sativum in der Stufentherapie der Hyperlipidamie. Med Welt 1993, 44:516-520.
• [171]Koch HP, Hahn G, Lawson L, Reuter HD, Siegers CP: Garlic-An introduction to the therapeutic application of Allium sativum L. Williams & Wilkins, Baltimore, im Druck 1995.
• [172]Eming SA, Piontek JO, Hunzelmann N, Rasokat H, Scharffetter-Kachanek K: Severe toxic contact dematitis caused by garlic. Br J Dermatol 1999, 141:391-392.
• [173]Falleroni AE, Zeiss CR, Levitz D: Occupational asthma secondary to inhalation of garlic dust. J Allergy Clin Immunol 1981, 68:156-160.
• [174]Papageogiou D, Corbet JP, Menezes F-Brando, Pecegueiro M, Benezra C: Allergic: contact dermatitis to garlic (Allium sativum I) identification of the allergens: the role of mono-, di- and trisulfides present in garlic. Arch Dermatol Res 1983, 275:229-234.
• [175]Rose KD, Croissant PD, Parliament CF, Levin MB: Spontaneous Spinal Epidural Hematoma with Associated Platelet Dysfunction from Excessive Garlic Ingestion: A case Report. Neurosurgery 1990, 26:880-882.
• [176]Sunter WH: Warfarin and garlic. Pharm J 1991, 246:722.
• [177]Burnham BE: Garlic as a possible risk for postoperative bleeding. Plast Recon Surg 1995, 95:213.
• [178]Fugh-Berman A: Herb-drug interactions. Lancet 2000, 355:134-138.
• [179]Petry JJ: Garlic and postoperative bleeding. Plastic Recon Surg 1995, 96:483-484.
• [180]Kendler BS: Garlic (Allium sativum) and onion (Allium cepa): a review of their relationship to cardiovascular disease. Prev Med 1987, 16(5):670-685.
• [181]Keys A: Wine, garlic and CHD in seven countries. Lancet 1980, 1(8160):145-146.