Full-length amelogenin influences the differentiation of human dental pulp stem cells

Stem Cell Research & Therapy

Matthias Folwaczny²  Michael Hristov¹  Reinhard Hickel²  Christian Diegritz²  Christina Ern²  Iris Frasheri² 

[1] Institute for Cardiovascular Prevention, Ludwig-Maximilian-University, Munich, Germany;Department of Conservative Dentistry and Periodontology, Ludwig-Maximilian-University, Goethestraße, 70, Munich, D-80336, Germany

关键词: Extracellular matrix;    Stem cells;    Gene expression;    Pulp biology;    Cell signaling;    Amelogenin;   

Others  :  1235629
DOI  :  10.1186/s13287-015-0269-9

 received in 2015-05-26, accepted in 2015-12-21,  发布年份 2016

【 摘 要 】

Background

Amelogenin is an extracellular matrix protein well known for its role in the organization and mineralization of enamel. Clinically, it is used for periodontal regeneration and, due to its finding also in predentin and intercellular spaces of dental pulp cells, it has recently been suggested for pulp capping procedures. The aim of this study was to analyse in vitro the effect of the recombinant human full-length amelogenin on the growth and differentiation of human dental pulp stem cells (hDPSCs).

Methods

Human DPSCs were treated with a supplement of amelogenin at a concentration of 10 ng/ml, 100 ng/ml and 1000 ng/ml. The groups were compared to the unstimulated control in terms of cell morphology and proliferation, mineralization and gene expression for ALP (alkaline phosphatase), DMP1 (dentin matrix protein-1) and DSPP (dentin sialophosphoprotein).

Results

Amelogenin affects hDPSCs differently than PDL (periodontal ligament) cells and other cell lines. The proliferation rate at two weeks is significantly reduced in presence of the highest concentration of amelogenin as compared to the unstimulated control. hDPSCs treated with low concentrations present a downregulation of DMP1 and DSPP, which is significant for DSPP (p = 0.011), but not for DMP1 (p = 0.395).

Conclusions

These finding suggest that the role of full-length amelogenin is not restricted to participation in tooth structure. It influences the differentiation of hDPSC according to various concentrations and this might impair the clinical results of pulp capping.

【 授权许可】

   
2016 Frasheri et al.

【 图 表 】

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