Emergence of human-like H3N2 influenza viruses in pet dogs in Guangxi, China

Virology Journal

Wei-Jian Huang³  Wen-Juan Yang³  Xiong Xiao³  Qing-Xiong Yu³  Guo-Jun Wang¹  Zu-Zhang Wei³  Hua-Bo Zhou²  Yan-Ning Mo³  Ying Chen³ 

[1] Department of Microbiology, Mount Sinai School of Medicine, New York 10029, NY, USA;Huabo Pet Medical Center, Yufeng District, No.342 Liushi Road, Liuzhou 545005, People’s Republic of China;College of Animal Science and Technology, Guangxi University, No.100 Daxue Road, Nanning 530004, People’s Republic of China

关键词: Human-like influenza viruses;    H3N2 subtype;    Pet dogs;   

Others  :  1131026
DOI  :  10.1186/s12985-015-0243-2

 received in 2014-07-13, accepted in 2015-01-19,  发布年份 2015

【 摘 要 】

Background

After the 1968 H3N2 pandemic emerged in humans, H3N2 influenza viruses continuously circulated and evolved in nature. An H3N2 variant was circulating in humans in the 1990s and subsequently introduced into the pig population in the 2000s. This virus gradually became the main subtype of swine influenza virus worldwide. However, there were no reports of infections in dogs with this virus.

Findings

In 2013, 35 nasal swabs from pet dogs were positive for Influenza A virus by RT-PCR. Two viruses were isolated and genetically characterized. In the phylogenetic trees of all gene segments, two H3N2 canine isolates clustered with Moscow/10/99 and most H3N2 swine influenza viruses. These results indicated that two H3N2 CIVs possessed high homology with human/swine influenza viruses, which at the same time exhibited some amino acid substitutions in NA, polymerase basic protein 1 (PB1), and nucleoprotein (NP), which probably were related to the interspecies transmission.

Conclusions

These two viruses share the highest homology with swine H3N2, Moscow/99-like viruses, which indicated that these viruses might originate from swine viruses.

【 授权许可】

   
2015 Chen et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Webster RG, Bean WJ, Gorman OT, Chambers TM, Kawaoka Y: Evolution and ecology of influenza A viruses. Microbiol Rev 1992, 56:152-79.
• [2]Crawford PC, Dubovi EJ, Castleman WL, Stephenson I, Gibbs EP, Chen L, et al.: Transmission of equine influenza virus to dogs. Science 2005, 310:482-5.
• [3]Lin D, Sun S, Du L, Ma J, Fan L, Pu J, et al.: Natural and experimental infection of dogs with pandemic H1N1/2009 influenza virus. J Gen Virol 2012, 93:119-23.
• [4]Song D, Lee C, Kang B, Jung K, Oh T, Kim H, et al.: Experimental infection of dogs with avian-origin canine influenza A virus (H3N2). Emerg Infect Dis 2009, 15:56-8.
• [5]Song D, Moon HJ, An DJ, Jeoung HY, Kim H, Yeom MJ, et al.: A novel reassortant canine H3N1 influenza virus between pandemic H1N1 and canine H3N2 influenza viruses in Korea. J Gen Virol 2012, 93:551-4.
• [6]Song QQ, Zhang FX, Liu JJ, Ling ZS, Zhu YL, Jiang SJ, et al.: Dog to dog transmission of a novel influenza virus (H5N2) isolated from a canine. Vet Microbiol 2013, 161:331-3.
• [7]Songserm T, Amonsin A, Jam-on R, Sae-Heng N, Pariyothorn N, Payungporn S, et al.: Fatal avian influenza A H5N1 in a dog. Emerg Infect Dis 2006, 12:1744-7.
• [8]Sun X, Xu X, Liu Q, Liang D, Li C, He Q, et al.: Evidence of avian-like H9N2 influenza A virus among dogs in Guangxi, China. Infect Genet Evol 2013, 20:471-5.
• [9]Horimoto T, Gen F, Murakami S, Iwatsuki-Horimoto K, Kato K, Akashi H, et al.: Serological evidence of infection of dogs with human influenza viruses in Japan. Vet Rec 2014, 174:96.
• [10]Ramirez-Martinez LA, Contreras-Luna M, De la Luz J, Manjarrez ME, Rosete DP, Rivera-Benitez JF, et al.: Evidence of transmission and risk factors for influenza A virus in household dogs and their owners. Influenza Other Respir Viruses 2013, 7:1292-6.
• [11]Sun Y, Shen Y, Zhang X, Wang Q, Liu L, Han X, et al.: A serological survey of canine H3N2, pandemic H1N1/09 and human seasonal H3N2 influenza viruses in dogs in China. Vet Microbiol 2014, 168:193-6.
• [12]Chang CP, New AE, Taylor JF, Chiang HS: Influenza virus isolations from dogs during a human epidemic in Taiwan. Int J Zoonoses 1976, 3:61-4.
• [13]Madhavan HN, Agarwal SC: Sero-epidemiology of human and canine influenza in Pondicherry, South India, during 1971–1974. Indian J Med Res 1976, 64:835-40.
• [14]Nikitin A, Cohen D, Todd JD, Lief FS: Epidemiological studies of A-Hong Kong-68 virus infection in dogs. Bull World Health Organ 1972, 47:471-9.
• [15]Fouchier RA, Bestebroer TM, Herfst S, Van Der Kemp L, Rimmelzwaan GF, Osterhaus AD: Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene. J Clin Microbiol 2000, 38:4096-101.
• [16]Hoffmann E, Stech J, Guan Y, Webster RG, Perez DR: Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol 2001, 146:2275-89.
• [17]Yu H, Zhang GH, Hua RH, Zhang Q, Liu TQ, Liao M, et al.: Isolation and genetic analysis of human origin H1N1 and H3N2 influenza viruses from pigs in China. Biochem Biophys Res Commun 2007, 356:91-6.
• [18]Matrosovich M, Zhou N, Kawaoka Y, Webster R: The surface glycoproteins of H5 influenza viruses isolated from humans, chickens, and wild aquatic birds have distinguishable properties. J Virol 1999, 73:1146-55.
• [19]Rogers GN, Paulson JC: Receptor determinants of human and animal influenza virus isolates: differences in receptor specificity of the H3 hemagglutinin based on species of origin. Virology 1983, 127:361-73.
• [20]Muranaka M, Yamanaka T, Katayama Y, Hidari K, Kanazawa H, Suzuki T, et al.: Distribution of influenza virus sialoreceptors on upper and lower respiratory tract in horses and dogs. J Vet Med Sci 2011, 73:125-7.
• [21]Ning ZY, Wu XT, Cheng YF, Qi WB, An YF, Wang H, et al.: Tissue distribution of sialic acid-linked influenza virus receptors in beagle dogs. J Vet Sci 2012, 13:219-22.