Maternal nutrient restriction in the ewe from early to midgestation programs reduced steroidogenic enzyme expression and tended to reduce progesterone content of corpora lutea, as well as circulating progesterone in nonpregnant aged female offspring

Reproductive Biology and Endocrinology

Stephen P Ford²  Peter W Nathanielsz¹  William J Murdoch²  Yan Ma²  Caleb O Lemley³  Lindsey A George²  Nuermaimaiti Tuersunjiang²  Nathan M Long⁴ 

[1] Department of Obstetrics and Gynecology, University of Texas Health Sciences Center, San Antonio, TX 78229, USA;Department of Animal Science, University of Wyoming, Laramie, WY 82071, USA;Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS 39762, USA;Department of Animal and Veterinary Sciences, Clemson University, Clemson, SC 29634, USA

关键词: Sheep;    Aged female offspring;    Altered progesterone levels;    Maternal nutrient restriction;    Fetal programming;   

Others  :  812009
DOI  :  10.1186/1477-7827-11-34

 received in 2013-01-18, accepted in 2013-05-02,  发布年份 2013

【 摘 要 】

Background

Previously we reported decreased circulating progesterone and fertility in one and two year old ewes born to undernourished mothers. This study was designed to investigate if this reduction in progesterone persisted into old age, and if it did, what mechanisms are involved.

Methods

Ewes were fed a nutrient restricted (NR, 50% of NRC recommendations) or control (C, 100% of NRC) diets from day 28 to 78 of gestation, then all were fed to requirements through parturition and weaning. Female offspring (4 per treatment group) were maintained as a group and fed to requirements from weaning until assigned to this study at 6 years of age. Ewes were synchronized for estrus (day 0) and blood samples were collected daily from day 0 to day 11 before necropsy on day 12. Blood serum and luteal tissue were assayed for progesterone concentrations by validated radioimmunoassay.

Results

Circulation progesterone concentrations tended to be lower (P = 0.06) in NR than C offspring from day 0 to 11 of the estrous cycle. While total luteal weight was similar across groups, total progesterone content also tended to be reduced (P = 0.07) in luteal tissue of NR than C offspring. Activity of hepatic progesterone catabolizing enzymes and selected angiogenic factors in luteal tissue were similar between groups. Messenger RNA expression of steroidogenic enzymes StAR and P450scc were reduced (P < 0.05), while protein expression of StAR tended to be reduced (P < 0.07) and P450scc was reduced (P < 0.05) in luteal tissue of NR versus C offspring.

Conclusions

There appears to be no difference in hepatic steroid catabolism that could have led to the decreased serum progesterone. However, these data are consistent with the programming of decreased steroidogenic enzyme expression in CL of NR offspring, leading to reduced synthesis and secretion of progesterone.

【 授权许可】

   
2013 Long et al.; licensee BioMed Central Ltd.

【 图 表 】

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