Toll-like receptor 3 gene polymorphisms and severity of pandemic A/H1N1/2009 influenza in otherwise healthy children

Virology Journal

Nicola Principi¹  Alessandro Plebani²  Emilio Fossali³  Claudio Pelucchi⁴  Laura Tagliaferri¹  Alessia Scala¹  Cinzia Mazza²  Silvia Giliani²  Claudio Giuseppe Molteni¹  Susanna Esposito¹ 

[1] Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Commenda 9, Milano, 20122, Italy;Nocivelli Institute for Molecular Medicine and Pediatric Clinic, University of Brescia, Brescia, Italy;Pediatric Emergency Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy;Department of Epidemiology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy

关键词: Toll-like receptors;    TLR3;    Pandemic A/H1N1/2009 influenza virus;    Influenza;    Innate immunity;    Children;   

Others  :  1153193
DOI  :  10.1186/1743-422X-9-270

 received in 2012-02-18, accepted in 2012-10-31,  发布年份 2012

【 摘 要 】

Background

Toll-like receptors (TLRs) form an essential part of the innate immune system, which plays a fundamental role in rapidly and effectively controlling infections and initiating adaptive immunity. There are no published data concerning the importance of polymorphisms of TLRs in conditioning susceptibility to influenza or the severity of the disease. The aim of this study was to evaluate whether selected polymorphisms of TLR2, TLR3 and TLR4 influence the incidence and clinical picture of pandemic A/H1N1/2009 influenza.

Results

The study involved 272 healthy children attending our Emergency Room for influenza-like illness (ILI), including 51 (18.8%) with pandemic A/H1N1/2009 influenza as revealed by real-time polymerase chain reaction, and 164 healthy controls examined after minor surgery. Genomic DNA was extracted from whole blood samples and five single-nucleotide polymorphisms (SNPs) were studied: TLR2 rs5743708, TLR3 rs5743313, TLR3 rs5743315, TLR4 rs4986790 and TLR4 rs4986791. The TLR3 rs5743313/CT polymorphism was found in all of the children with pneumonia and influenza infection, but in a significantly smaller number of those with A/H1N1/2009 influenza without pneumonia (<0.0001). TLR2, TLR3 rs5743315/AC and TLR4 polymorphisms were equally distributed in all of the groups regardless of the presence of the pandemic A/H1N1/2009 virus and clinical diagnosis. Viral load was comparable in all of the study groups.

Conclusions

There is a close relationship between the presence of TLR3 rs5743313/CT and an increased risk of pneumonia in children infected by the pandemic A/H1N1/2009 influenza virus.

【 授权许可】

   
2012 Esposito et al.; licensee BioMed Central Ltd.

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