SET protein up-regulated testosterone production in the cultured preantral follicles

Reproductive Biology and Endocrinology

Jiayin Liu¹  Feiyang Diao¹  Xiang Ma¹  Yuan Zhang¹  Mei Li¹  Xue Dai²  Li Gao¹  Yugui Cui¹  Lingling Gao¹  Boqun Xu² 

[1] The State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing, 210029, China;Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, China

关键词: Preantral follicles;    Androgen production;    SET;   

Others  :  828823
DOI  :  10.1186/1477-7827-11-9

 received in 2012-11-16, accepted in 2013-02-07,  发布年份 2013

【 摘 要 】

Background

We found previously that the expression of SET gene was up-regulated in polycystic ovaries. Evidences suggested that SET protein was essential for regulating both the promoter activity of CYP17A1 and the biological activity of P450c17. In this study, we explored whether SET regulated androgen production in preantral follicles.

Methods

The mouse preantral follicles were cultured in vitro. Testosterone secretion and expression of steroidogenic enzymes were observed in the preantral follicles treated in vitro by SET overexpression and knockdown.

Results

Testosterone levels in the media of the AdCMV-SET infected follicles significantly increased, and the CYP17A1 and HSD3B2 expression also significantly increased (P < 0.05). Testosterone levels in AdSiRNA-SET infected group decreased, and so did CYP17A1 and HSD3B2 expression (P < 0.05).

Conclusions

SET played a positive role in regulating ovarian androgen biosynthesis by enhancing the transcription of steroidogenic enzymes CYP17A1 and HSD3B2, which maybe contribute to the hyperandrogenism in PCOS.

【 授权许可】

   
2013 Xu et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 2.

Figure 3.

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