Comparative proteomics analysis of Spodoptera frugiperda cells during Autographa californica multiple nucleopolyhedrovirus infection

Virology Journal

Dongling Wen¹  Qin Wang¹  Zhiqiang Chen¹  Jianliang Liu¹  Hang Gao¹  Youhua Xiong¹  Qian Yu¹ 

[1] College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District 510225, Guangdong, People’s Republic of China

关键词: KEGG;    GO annotation;    Proteomics analysis;    iTRAQ;    Spodoptera frugiperda cell;    Autographa californica multiple nucleopolyhedrovirus;   

Others  :  1224533
DOI  :  10.1186/s12985-015-0346-9

 received in 2015-04-02, accepted in 2015-07-21,  发布年份 2015

【 摘 要 】

Background

Increasing evidence sugggest that in addition of balculovirus controling insect host, host cells also responds to balculovirus infection. However, compared to existing knowledge on virus gene, host cell responses are relatively poorly understood.

Methods

In this study, Spodoptera frugiperda (Sf9) cells were infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV). The protein composition and protein changes of Spodoptera frugiperda (Sf9) cells of different infection stages were analysed by isobaric tag for relative and absolute quantification (iTRAQ) techniques.

Results

A total of 4004 Sf9 proteins were identified by iTRAQ and 413 proteins were found as more than 1.5-fold changes in abundance. The 413 proteins were categorised according to GO classification for insects and were categorised into: biological process, molecular function and cellular component.

Conclusions

The determination of the protein changes in infected Sf9 cells would help to better understanding of host cell responses and facilitate better design of this virus-host cell interaction in pest insect control and other related fields.

【 授权许可】

   
2015 Yu et al.

【 图 表 】

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