Detection and genetic characterization of Seoul Virus from commensal brown rats in France

Virology Journal

Lorraine M McElhinney¹  Catherine Legras-Lachuer⁴  Michel Pépin²  Anthony R Fooks¹  Joel Lachuer⁴  Philippe Marianneau³  Marc Artois²  Nicolas Nazaret⁴  Katja Voller⁵  Fabienne Barbet⁴  Catherine Rey⁴  Sandra Lacôte³  Denise A Marston⁵  Maria-Halima Laaberki²  Florence Ayral²  Kieran C Pounder⁶  Tatiana Dupinay² 

[1] National Consortium for Zoonosis Research, University of Liverpool, Leahurst, Neston, South Wirral CH64 7TE, UK;Université de Lyon, VetAgro Sup, USC 1233/Equipe « Pathogènes émergents et rongeurs sauvages (PERS), F-69280 Marcy-L’Etoile, France;Anses-Laboratoire de Lyon, Unité Virologie, F-69347 Lyon, France;Viroscan 3D / Profilexpert, Faculté de Médecine et de Pharmacie, 3453 CNRS-US7 Inserm, 8 avenue Rockefeller, aile C2, 69373, Lyon Cedex 08, France;Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal Health and Veterinary Laboratories Agency (AHVLA), New Haw, Addlestone, Surrey KT15 3NB, UK;Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK

关键词: Viral enrichment;    Next generation sequencing;    Rattus norvegicus;    Brown rat;    France;    SEOV;    Hantavirus;   

Others  :  810943
DOI  :  10.1186/1743-422X-11-32

 received in 2013-10-30, accepted in 2014-01-27,  发布年份 2014

【 摘 要 】

Background

Hantaviruses are single-stranded RNA viruses, which are transmitted to humans primarily via inhalation of aerosolised virus in contaminated rodent urine and faeces. Whilst infected reservoir hosts are asymptomatic, human infections can lead to two clinical manifestations, haemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS), with varying degrees of clinical severity. The incidence of rodent and human cases of Seoul virus (SEOV) in Europe has been considered to be low, and speculated to be driven by the sporadic introduction of infected brown rats (Rattus norvegicus) via ports.

Methods

Between October 2010 and March 2012, 128 brown rats were caught at sites across the Lyon region in France.

Results

SEOV RNA was detected in the lungs of 14% (95% CI 8.01 – 20.11) of brown rats tested using a nested pan-hantavirus RT-PCR (polymerase gene). Phylogenetic analysis supports the inclusion of the Lyon SEOV within Lineage 7 with SEOV strains originating from SE Asia and the previously reported French & Belgian SEOV strains. Sequence data obtained from the recent human SEOV case (Replonges) was most similar to that obtained from one brown rat trapped in a public park in Lyon city centre. We obtained significantly improved recovery of virus genome sequence directly from SEOV infected lung material using a simple viral enrichment approach and NGS technology.

Conclusions

The detection of SEOV in two wild caught brown rats in the UK and the multiple detection of SEOV infected brown rats in the Lyon region of France, suggests that SEOV is circulating in European brown rats. Under-reporting and difficulties in identifying the hantaviruses associated with HFRS may mask the public health impact of SEOV in Europe.

【 授权许可】

   
2014 Crown copyright; licensee BioMed Central Ltd.

【 图 表 】

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