Enhanced epithelial to mesenchymal transition (EMT) and upregulated MYC in ectopic lesions contribute independently to endometriosis

Reproductive Biology and Endocrinology

Heinrich Husslein²  Berthold Streubel³  Rene Wenzl³  Gülen Yerlikaya³  Erika Marton³  Nadine Nirtl³  Susanne Gamperl³  Peter Birner¹  Katharina Proestling³ 

[1]Department of Pathology, Medical University of Vienna, Waehringer Guertel 18-20, Vienna 1090, Austria

[2]Department of Obstetrics and Gynecology, St. Michael’s Hospital, University of Toronto, 30 Bond street, Toronto M5B 1 W8, Ontorio, Canada

[3]Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, Vienna 1090, Austria

关键词: EMT;    SLUG;    SNAIL;    TWIST1;    MYC;    Endometriosis;   

Others  :  1224340
DOI  :  10.1186/s12958-015-0063-7

 received in 2015-01-29, accepted in 2015-06-09,  发布年份 2015

【 摘 要 】

Background

Epithelial to mesenchymal transition (EMT) is a process in which epithelial cells lose polarity and cell-to-cell contacts and acquire the migratory and invasive abilities of mesenchymal cells. These abilities are thought to be prerequisites for the establishment of endometriotic lesions. A hallmark of EMT is the functional loss of E-cadherin (CDH1) expression in epithelial cells. TWIST1, a transcription factor that represses E-cadherin transcription, is among the EMT inducers. SNAIL, a zinc-finger transcription factor, and its close relative SLUG have similar properties to TWIST1 and are thus also EMT inducers. MYC, which is upregulated by estrogens in the uterus by an estrogen response cis-acting element (ERE) in its promoter, is associated with proliferation in endometriosis. The role of EMT and proliferation in the pathogenesis of endometriosis was evaluated by analyzing TWIST1, CDH1 and MYC expression.

Methods

CDH1, TWIST1, SNAIL and SLUG mRNA expression was analyzed by qRT-PCR from 47 controls and 74 patients with endometriosis. Approximately 42 ectopic and 62 eutopic endometrial tissues, of which 30 were matched samples, were collected during the same surgical procedure. We evaluated TWIST1 and MYC protein expression by immunohistochemistry (IHC) in the epithelial and stromal tissue of 69 eutopic and 90 ectopic endometrium samples, of which 49 matched samples were analyzed from the same patient. Concordant expression of TWIST1/SNAIL/SLUG and CDH1 but also of TWIST1 and MYC was analyzed.

Results

We found that TWIST1, SNAIL and SLUG are overexpressed (p < 0.001, p = 0.016 and p < 0.001) in endometriosis, while CDH1 expression was concordantly reduced in these samples (p < 0.001). Similar to TWIST1, the epithelial expression of MYC was also significantly enhanced in ectopic endometrium compared to eutopic tissues (p = 0.008). We found exclusive expression of either TWIST1 or MYC in the same samples (p = 0.003).

Conclusions

Epithelial TWIST1 is overexpressed in endometriosis and may contribute to the formation of endometriotic lesions by inducing epithelial to mesenchymal transition, as CDH1 was reduced in ectopic lesions. We found exclusive expression of either TWIST1 or MYC in the same samples, indicating that EMT and proliferation contribute independently of each other to the formation of endometriotic lesions.

【 授权许可】

   
2015 Proestling et al.

【 图 表 】

Figure 2.

Fig. 2.

Fig. 3.

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