Duck Tembusu virus exhibits neurovirulence in BALB/c mice

Virology Journal

Jingliang Su¹  Bo Han¹  Wenliang Su¹  Kegong Tian³  Xiuling Yu³  Yongyue Wang¹  Lijiao Zhang¹  Xiaoxia Li³  Shuang Li² 

[1] Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;Innovation Research Institute, Beijing Vica Group, Beijing 100085, China;Veterinary Diagnostic Laboratory, China Animal Disease Control Center, Beijing 100193, China

关键词: BALB/c mice;    Neurovirulence;    Flavivirus;    Duck Tembusu virus;   

Others  :  826135
DOI  :  10.1186/1743-422X-10-260

 received in 2013-02-13, accepted in 2013-08-06,  发布年份 2013

【 摘 要 】

Background

Duck Tembusu virus is a member of the Ntaya group in the genus Flavivirus. The virus has been responsible for severe duck egg-drop syndrome in China since 2010. Its emergence and rapid spread have caused great economic loss for the poultry industry. The epidemiology of the virus infection and the potential threat to public health is of great concern because of the infective and zoonotic nature of flaviviruses.

Results

In this study, the pathogenicity of duck Tembusu virus in BALB/c mice was investigated. Infected mice developed clinical signs, including loss of appetite, ruffled hair, weight loss, disorientation, blindness and paralysis of hind limbs from six days post- infection following intracerebral inoculation. Morbidity was 100%, with mortality ranging from 20 to 80% in three- to eight-week-old mice. High virus titers were recovered from the brain, and the virus was distributed in several organs. Histologically, there was widespread non-suppurative encephalitis in the brain. Lymphocyte depletion in the spleen was observed, along with fatty degeneration in the liver and kidney.

Conclusions

Our results demonstrate, for the first time, that duck Tembusu virus is highly neurovirulent in BALB/c mice. The mouse model used in this work was able to produce Tembusu virus infection and could be useful for elucidating some of the aspects of the pathophysiology of other flavivirus infections.

【 授权许可】

   
2013 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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