Human and murine APOBEC3s restrict replication of koala retrovirus by different mechanisms

Retrovirology

Hung Fan¹  Takayuki Miyazawa²  Felipe Galvez¹  Dat Ha¹  Takayuki Nitta³ 

[1] Cancer Research Institute, University of California, Irvine, Irvine 92697-3905, CA, USA;Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan;Department of Biology, Savannah State University, 3219 College St, Savannah 31404-5254, GA, USA

关键词: Glyco-gag;    APOBEC3;    KoRV;   

Others  :  1223838
DOI  :  10.1186/s12977-015-0193-1

 received in 2015-02-17, accepted in 2015-07-23,  发布年份 2015

【 摘 要 】

Background

Koala retrovirus (KoRV) is an endogenous and exogenous retrovirus of koalas that may cause lymphoma. As for many other gammaretroviruses, the KoRV genome can potentially encode an alternate form of Gag protein, glyco-gag.

Results

In this study, a convenient assay for assessing KoRV infectivity in vitro was employed: the use of DERSE cells (initially developed to search for infectious xenotropic murine leukemia-like viruses). Using infection of DERSE and other human cell lines (HEK293T), no evidence for expression of glyco-gag by KoRV was found, either in expression of glyco-gag protein or changes in infectivity when the putative glyco-gag reading frame was mutated. Since glyco-gag mediates resistance of Moloney murine leukemia virus to the restriction factor APOBEC3, the sensitivity of KoRV (wt or putatively mutant for glyco-gag) to restriction by murine (mA3) or human APOBEC3s was investigated. Both mA3 and hA3G potently inhibited KoRV infectivity. Interestingly, hA3G restriction was accompanied by extensive G → A hypermutation during reverse transcription while mA3 restriction was not. Glyco-gag status did not affect the results.

Conclusions

These results indicate that the mechanisms of APOBEC3 restriction of KoRV by hA3G and mA3 differ (deamination dependent vs. independent) and glyco-gag does not play a role in the restriction.

【 授权许可】

   
2015 Nitta et al.

【 图 表 】

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