Nanochelating based nanocomplex, GFc7, improves quality and quantity of human mesenchymal stem cells during in vitro expansion

Stem Cell Research & Therapy

Masoud Soleimani³  Mohammad Hassan Nazaran²  Zahra Masoumi¹  Saideh Fakharzadeh²  Somayeh Kalanaky²  Amir Atashi³  Atena Hajarizadeh¹  Maryam Hafizi² 

[1] Stem Cell Technology Research Center, Tehran, Iran;Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran;Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

关键词: Nanochelating technology;    Nanocomplex;    Human mesenchymal stem cells;    GFc7;    Expansion;   

Others  :  1235101
DOI  :  10.1186/s13287-015-0216-9

 received in 2015-04-25, accepted in 2015-10-28,  发布年份 2015

【 摘 要 】

Introduction

Human mesenchymal stem cells (hMSCs) have been approved for therapeutic applications. Despite the advances in this field, in vitro approaches are still required to improve the essential indices that would pave the way to a bright horizon for an efficient transplantation in the future. Nanotechnology could help to improve these approaches. Studies signified the important role of iron in stem cell metabolism and efficiency of copper chelation application for stem cell expansion 

Methods

For the first time, based on novel Nanochelating technology, we design an iron containing copper chelator nano complex, GFc7 and examined on hMSCs during in vitro expansion. In this study, the hMSCs were isolated, characterized and expanded in vitro in two media (with or without GFc7). Then proliferation, cell viability, cell cycle analysis, surface markers, HLADR, pluripotency genes expression, homing and antioxidative defense at genes and protein expression were investigated. Also we analyzed the spontaneous differentiation and examined osteogenic and lipogenic differentiation. 

Results

GFc7 affected the expression of key genes, improving both the stemness and fitness of the cells in a precise and balanced manner. We observed significant increases in cell proliferation, enhanced expression of pluripotency genes and homing markers, improved antioxidative defense, repression of genes involved in spontaneous differentiation and exposing the hMSCs to differentiation medium indicated that pretreatment with GFc7 increased the quality and rate of differentiation.

Conclusions

Thus, GFc7 appears to be a potential new supplement for cell culture medium for increasing the efficiency of transplantation.

【 授权许可】

   
2015 Hafizi et al.

【 图 表 】

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