HyperART: non-invasive quantification of leaf traits using hyperspectral absorption-reflectance-transmittance imaging

Plant Methods

Uwe Rascher³  Hanno Scharr³  Marcus Jansen²  Maria Pilar Cendrero-Mateo³  Simone Schmittgen³  Dimitrios Fanourakis¹  Sergej Bergsträsser³ 

[1] Present address: Institute of Viticulture, Floriculture and Vegetable Crops, Hellenic Agricultural Organization ‘Demeter’ (NAGREF), Heraklio, GR 71003, Greece;Present address: LemnaTec GmbH, Pascalstraße 59, Aachen, 52076, Germany;Institute for Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich GmbH, Jülich, 52425, Germany

关键词: Imaging spectroscopy;    Transmittance;    Reflectance;    Non-invasive phenotyping;    Hyperspectral imaging;    FluoWat;    FieldSpec;    Chlorophyll content;    Cercospora beticola;    Absorption;   

Others  :  1132153
DOI  :  10.1186/s13007-015-0043-0

 received in 2014-10-11, accepted in 2015-01-03,  发布年份 2015

【 摘 要 】

Background

Combined assessment of leaf reflectance and transmittance is currently limited to spot (point) measurements. This study introduces a tailor-made hyperspectral absorption-reflectance-transmittance imaging (HyperART) system, yielding a non-invasive determination of both reflectance and transmittance of the whole leaf. We addressed its applicability for analysing plant traits, i.e. assessing Cercospora beticola disease severity or leaf chlorophyll content. To test the accuracy of the obtained data, these were compared with reflectance and transmittance measurements of selected leaves acquired by the point spectroradiometer ASD FieldSpec, equipped with the FluoWat device.

Results

The working principle of the HyperART system relies on the upward redirection of transmitted and reflected light (range of 400 to 2500 nm) of a plant sample towards two line scanners. By using both the reflectance and transmittance image, an image of leaf absorption can be calculated. The comparison with the dynamically high-resolution ASD FieldSpec data showed good correlation, underlying the accuracy of the HyperART system. Our experiments showed that variation in both leaf chlorophyll content of four different crop species, due to different fertilization regimes during growth, and fungal symptoms on sugar beet leaves could be accurately estimated and monitored. The use of leaf reflectance and transmittance, as well as their sum (by which the non-absorbed radiation is calculated) obtained by the HyperART system gave considerably improved results in classification of Cercospora leaf spot disease and determination of chlorophyll content.

Conclusions

The HyperART system offers the possibility for non-invasive and accurate mapping of leaf transmittance and absorption, significantly expanding the applicability of reflectance, based on mapping spectroscopy, in plant sciences. Therefore, the HyperART system may be readily employed for non-invasive determination of the spatio-temporal dynamics of various plant properties.

【 授权许可】

   
2015 Bergsträsser et al.; licensee BioMed Central.

【 图 表 】

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