Rapid detection of Akabane virus by a novel reverse transcription loop-mediated isothermal amplification assay (RT-LAMP)

Virology Journal

Chuangfu Chen¹  Xuepeng Cai²  Zaichao Zhang¹  Haibo Yang¹  Zhihao He¹  Yucheng Liu¹  Guochao Wang¹  Qingling Meng²  Junwei Wang¹  Jun Qiao¹ 

[1] College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, People’s Republic of China;State Key Lab of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, People’s Republic of China

关键词: Reverse transcription loop-mediated isothermal amplification assay;    Akabane virus;    Rapid detection;   

Others  :  821434
DOI  :  10.1186/1743-422X-10-288

 received in 2013-01-24, accepted in 2013-07-22,  发布年份 2013

【 摘 要 】

Background

Akabane disease, caused by Akabane virus, is an insect-transmitted disease of ruminants that is primarily characterized by fetal damage.

Methods and results

In this study, a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of Akabane virus was successfully developed. The primers were designed to target the highly conserved fragment of nucleoprotein from the Akabane virus. The results indicate that the assay is highly specific and sensitive with a detection limit of 5.0 TCID₅₀ /mL within a 60-min incubation time. A total of 126 abortive samples collected from Xinjiang province were detected by the established RT-LAMP. The results of RT-LAMP assay showed 96.8% agreement with the semi-nested RT-PCR.

Conclusion

This study is to first to develop a rapid, sensitive, and accurate method for the detection of Akabane virus, which may be used to screen clinical samples in developing countries or regions.

【 授权许可】

   
2013 Qiao et al.; licensee BioMed Central Ltd.

【 图 表 】

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