High prevalence of hepatitis B virus genotype C/C1 in the Minangkabau ethnic group in Indonesia

Virology Journal

George Mathew²  Nasrul Zubir¹  Widyarman S Rasman³  Erlys B Julianto³  Szeifoul Afadlal²  Febi Andriani²  Tri S Kurniasih²  Mariana DB Intan²  Ismail Fanany²  Arnelis¹  Rama Dhenni²  Andi Utama²  Marlinang D Siburian² 

[1] Department of Internal Medicine, M. Djamil Hospital, Padang, Indonesia;Molecular Epidemiology Division, Mochtar Riady Institute for Nanotechnology, Universitas Pelita Harapan, Lippo Karawaci, Tangerang, Indonesia;Red Cross Unit, Padang, Indonesia

关键词: Minangkabau;    BCP mutation;    Pre-S mutation;    HBV genotype;   

Others  :  1152172
DOI  :  10.1186/1743-422X-10-27

 received in 2012-07-08, accepted in 2013-01-15,  发布年份 2013

【 摘 要 】

Background

The Minangkabau is one of the major ethnic groups in Indonesia. Previous studies with a limited number of samples have shown a different prevalence of HBV/C in the Minangkabau compared to the Indonesian population in general. The aim of this study was to assess the HBV genotype distribution pattern and the prevalence of pre-S, T1753V and A1762T/G1764A mutations among the Minangkabau HBV carriers. The samples were collected from Padang, West Sumatera and from western Java. Mixed primers for specific genotypes were used to determine the HBV genotype. Pre-S or S genes were amplified, sequenced and aligned with reference sequences from GenBank to derive a phylogenetic tree for subgenotyping. Pre-S genes were also analyzed for mutations. The basal core promoter (BCP) region was amplified and directly sequenced to analyze T1753V and A1762T/G1764A mutations.

Results

The predominant HBV genotype among the Minangkabau HBV carriers (n=117) was C (72.6%) followed by B (24.8%) and co-infection with B and C (2.6%). The prevalence of pre-S mutations, including both the pre-S deletion and pre-S2 start codon mutation, was 41.0%, and the T1753V and A1762T/G1764A mutations were found in 51.9% and 71.2% respectively. HBV/C1 was the predominant HBV subgenotype in the Minangkabau HBV carriers, and was found in 66.2%, followed by B3, B7, C8, B2, B9, C2, and C10 (18.3%, 7.0%, 2.8%, 1.4%, 1.4%, 1.4%, and 1.4% respectively). From samples that were found to be co-infected with HBV B and C, two samples were successfully cloned and subgenotyped, including one with mixed subgenotypes of B3 and C1, and another one with mixed subgenotypes of B7, C1, putative intergenotypic of B/A, and C/A. Furthermore, three samples from donors of non-Minangkabau ethnicity from Padang were found to be infected with an intragenotypic recombination form, including a putative recombinant of B8/B3 and B9/B7.

Conclusion

HBV/C with subgenotype C1 was the predominant HBV genotype among HBV carriers of Minangkabau ethnicity. The prevalence of pre-S, A1762T/G1764A, and T1753V mutations was higher among the Minangkabau compared to Indonesian HBV carriers in general.

【 授权许可】

   
2013 Siburian et al.; licensee BioMed Central Ltd.

【 图 表 】

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