Magnetic bead-based salivary peptidome profiling for periodontal-orthodontic treatment

Proteome Science

Jiuxiang Lin¹  Feng Chen²  Ning Du²  Yanheng Zhou¹  Xuedong Wang¹  Hui Zheng¹  Tian Cao¹  Ruoxuan Li³  Shaonan Zhou¹  Jieni Zhang¹ 

[1] Departments of Orthodontics and Central Laboratory, School of Stomatology, Peking University, #22 Zhongguancun South Road, Haidian District, Beijing, 100081, People's Republic of China;Department of Central Laboratory, School of Stomatology, Peking University, Beijing, 100081, People's Republic of China;Department of Stomatology, Beijing An Zhen Hospital, Capital University of Medical Sciences, Beijing, 100081, People's Republic of China

关键词: MALDI-TOF MS;    Saliva;    Peptidome;    Periodontal-orthodontic treatment;   

Others  :  817156
DOI  :  10.1186/1477-5956-10-63

 received in 2012-07-05, accepted in 2012-10-23,  发布年份 2012

【 摘 要 】

Background

Patients with periodontitis seek periodontal-orthodontic treatment to address certain functional and aesthetic problems. However, little is known of the effect of periodontitis on orthodontic treatment. Thus, we compared the differences in peptide mass fingerprints of orthodontic patients with and without periodontitis by MALDI-TOF MS using a magnetic bead-based peptidome analysis of saliva samples. In this way, we aimed to identify and explore a panel of differentially-expressed specific peptides.

Results

Saliva samples from 24 patients (eight orthodontic patients without periodontitis, eight with periodontitis and another eight with periodontitis but no orthodontic treatment) were analyzed, and peptide mass fingerprints were created by scanning MS signals using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with magnetic beads. Nine mass peaks showed significant differences. Orthodontic patients in the group without periodontal disease showed higher mass peaks for seven peptides of the nine, whereas the mass peaks for the other two peptides were higher in the periodontal-orthodontic patients. Besides, these differentially-expressed peptides were sequenced.

Conclusions

The elucidated candidate biomarkers indicated interactions between periodontal condition and orthodontic treatment and their contributions to the changes of saliva protein profiles. Our results provide novel insight into the altered salivary protein profile during periodontal-orthodontic treatment, and may lead to the development of a therapeutic monitoring strategy for periodontics and orthodontics.

【 授权许可】

   
2012 Zhang et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Kim YI, Kim MJ, Choi JI, Park SB: A multidisciplinary approach for the management of pathologic tooth migration in a patient with moderately advanced periodontal disease. Int J Periodontics Restorative Dent 2012, 32:225-230.
• [2]Zetu I, Ogodescu E, Zetu L, Stratul SI, Rusu D, Talpos S, Ogodescu A: [An interdisciplinary approach to complex orthodontic-periodontal clinical situations in adult patients]. Rev Med Chir Soc Med Nat Iasi 2011, 115:1262-1266.
• [3]Tew JG, El Shikh ME, El Sayed RM, Schenkein HA: Dendritic cells, antibodies reactive with oxLDL, and inflammation. J Dent Res 2012, 91:8-16.
• [4]Dersot JM: Gingival recession and adult orthodontics: a clinical evidence-based treatment proposal. Int Orthod 2012, 10:29-42.
• [5]Beader N, Ivic-Kardum M: The role of cytomegalovirus infection in the pathogenesis of periodontal diseases. Acta Clin Croat 2011, 50:61-66.
• [6]Tezal M, Uribe S: A lack of consensus in the measurement methods for and definition of periodontitis. J Am Dent Assoc 2011, 142:666-667.
• [7]Bader HI: Salivary diagnostics in medicine and dentistry: a review. Dent Today 2011, 30:46-48. 50–41; quiz 52–43.
• [8]Rosa N, Correia MJ, Arrais JP, Lopes P, Melo J, Oliveira JL, Barros M: From the salivary proteome to the OralOme: comprehensive molecular oral biology. Arch Oral Biol 2012, 57:853-864.
• [9]Punyadeera C, Dimeski G, Kostner K, Beyerlein P, Cooper-White J: One-step homogeneous C-reactive protein assay for saliva. J Immunol Methods 2011, 371:19-25.
• [10]Punyadeera C: Human saliva as a tool to investigate intimate partner violence. Brain Behav Immun 2012, 26:541-542.
• [11]Li Y, Denny P, Ho CM, Montemagno C, Shi W, Qi F, Wu B, Wolinsky L, Wong DT: The Oral Fluid MEMS/NEMS Chip (OFMNC): diagnostic and translational applications. Adv Dent Res 2005, 18:3-5.
• [12]Schulz BL, Cooper-White J, Punyadeera C: Saliva proteome research: current status and future outlook. Crit Rev Biotechnol 2012. [Epub ahead of print].
• [13]Zhang L, Xiao H, Karlan S, Zhou H, Gross J, Elashoff D, Akin D, Yan X, Chia D, Karlan B, Wong DT: Discovery and preclinical validation of salivary transcriptomic and proteomic biomarkers for the non-invasive detection of breast cancer. PLoS One 2010, 5:e15573.
• [14]Ferraccioli G, De Santis M, Peluso G, Inzitari R, Fanali C, Bosello SL, Iavarone F, Castagnola M: Proteomic approaches to Sjogren's syndrome: a clue to interpret the pathophysiology and organ involvement of the disease. Autoimmun Rev 2010, 9:622-626.
• [15]Giusti L, Baldini C, Ciregia F, Giannaccini G, Giacomelli C, De Feo F, Delle Sedie A, Riente L, Lucacchini A, Bazzichi L, Bombardieri S: Is GRP78/BiP a potential salivary biomarker in patients with rheumatoid arthritis? Proteomics Clin Appl 2010, 4:315-324.
• [16]Wu JY, Yi C, Chung HR, Wang DJ, Chang WC, Lee SY, Lin CT, Yang YC, Yang WC: Potential biomarkers in saliva for oral squamous cell carcinoma. Oral Oncol 2010, 46:226-231.
• [17]Hu S, Arellano M, Boontheung P, Wang J, Zhou H, Jiang J, Elashoff D, Wei R, Loo JA, Wong DT: Salivary proteomics for oral cancer biomarker discovery. Clin Cancer Res 2008, 14:6246-6252.
• [18]Vitorino R, de Morais Guedes S, Ferreira R, Lobo MJ, Duarte J, Ferrer-Correia AJ, Tomer KB, Domingues PM, Amado FM: Two-dimensional electrophoresis study of in vitro pellicle formation and dental caries susceptibility. Eur J Oral Sci 2006, 114:147-153.
• [19]Szabo GT, Tihanyi R, Csulak F, Jambor E, Bona A, Szabo G, Mark L: Comparative Salivary Proteomics of Cleft Palate Patients. Cleft Palate Craniofac J 2011, 48:708-716.
• [20]Goncalves Lda R, Soares MR, Nogueira FC, Garcia C, Camisasca DR, Domont G, Feitosa AC, Pereira Dde A, Zingali RB, Alves G: Comparative proteomic analysis of whole saliva from chronic periodontitis patients. J Proteomics 2010, 73:1334-1341.
• [21]Al-Tarawneh SK, Bencharit S: Applications of Surface-Enhanced Laser Desorption/Ionization Time-Of-Flight (SELDI-TOF) Mass Spectrometry in Defining Salivary Proteomic Profiles. Open Dent J 2009, 3:74-79.
• [22]Schaub NP, Jones KJ, Nyalwidhe JO, Cazares LH, Karbassi ID, Semmes OJ, Feliberti EC, Perry RR, Drake RR: Serum proteomic biomarker discovery reflective of stage and obesity in breast cancer patients. J Am Coll Surg 2009, 208:970-978. discussion 978–980.
• [23]Schwamborn K, Krieg RC, Grosse J, Reulen N, Weiskirchen R, Knuechel R, Jakse G, Henkel C: Serum proteomic profiling in patients with bladder cancer. Eur Urol 2009, 56:989-996.
• [24]Derton N, Derton R, Perini A, Gracco A, Fornaciari PA: Orthodontic treatment in periodontal patients: a case report with 7 years follow-up. Int Orthod 2011, 9:92-109.
• [25]Lee JW, Lee SJ, Lee CK, Kim BO: Orthodontic treatment for maxillary anterior pathologic tooth migration by periodontitis using clear aligner. J Periodontal Implant Sci 2011, 41:44-50.
• [26]Boyer S, Fontanel F, Danan M, Olivier M, Bouter D, Brion M: Severe periodontitis and orthodontics: evaluation of long-term results. Int Orthod 2011, 9:259-273.
• [27]van Gastel J, Teughels W, Quirynen M, Struyf S, Van Damme J, Coucke W, Carels C: Longitudinal changes in gingival crevicular fluid after placement of fixed orthodontic appliances. Am J Orthod Dentofacial Orthop 2011, 139:735-744.
• [28]Hans M, Hans VM: Toll-like receptors and their dual role in periodontitis: a review. J Oral Sci 2011, 53:263-271.
• [29]Al-Tarawneh SK, Border MB, Dibble CF, Bencharit S: Defining salivary biomarkers using mass spectrometry-based proteomics: a systematic review. OMICS 2011, 15:353-361.
• [30]Yang J, Song YC, Dang CX, Song TS, Liu ZG, Guo YM, Li ZF, Huang C: Serum peptidome profiling in patients with gastric cancer. Clin Exp Med 2011, 12(2):79-87.
• [31]Wu ZZ, Wang JG, Zhang XL: Diagnostic model of saliva protein finger print analysis of patients with gastric cancer. World J Gastroenterol 2009, 15:865-870.
• [32]Mizuno N, Niitani M, Shiba H, Iwata T, Hayashi I, Kawaguchi H, Kurihara H: Proteome analysis of proteins related to aggressive periodontitis combined with neutrophil chemotaxis dysfunction. J Clin Periodontol 2011, 38:310-317.
• [33]Rangé H, Léger T, Huchon C, Ciangura C, Diallo D, Poitou C, Meilhac O, Bouchard P, Chaussain C: Salivary proteome modifications associated with periodontitis in obese patients. J Clin Periodontol 2012, 39:799-806.
• [34]Zhang J, Zhou S, Zheng H, Zhou Y, Chen F, Lin J: Magnetic bead-based salivary peptidome profiling analysis during orthodontic treatment durations. Biochem Biophys Res Commun 421:844-849.
• [35]O'Brien M: The reciprocal relationship between inflammation and coagulation. Top Companion Anim Med 2012, 27:46-52.
• [36]Matsuyama T, Tokuda M, Izumi Y: Significance of thrombomodulin release from gingival epithelial cells in periodontitis patients. J Periodontal Res 2008, 43:379-385.
• [37]Inomata M, Ishihara Y, Matsuyama T, Imamura T, Maruyama I, Noguchi T, Matsushita K: Degradation of vascular endothelial thrombomodulin by arginine- and lysine-specific cysteine proteases from Porphyromonas gingivalis. J Periodontol 2009, 80:1511-1517.
• [38]Amado F, Lobo MJ, Domingues P, Duarte JA, Vitorino R: Salivary peptidomics. Expert Rev Proteomics 2010, 7:709-721.
• [39]Pfaffe T, Cooper-White J, Beyerlein P, Kostner K, Punyadeera C: Diagnostic Potential of Saliva: Current State and Future Applications. Clin Chem 2011, 57:675-687.