In vitro pre-vascularisation of tissue-engineered constructs A co-culture perspective

Vascular Cell

Dietmar Werner Hutmacher⁴  Eugen Bogdan Petcu⁵  Saso Ivanovski⁵  Kiarash Khosrotehrani³  Elena M De-Juan-Pardo⁴  Ulrich Bonda¹  Mélanie Antille²  Jeremy Baldwin⁴ 

[1] Leibniz Institute of Polymer Research Dresden (IPF) & Max Bergmann Center of Biomaterials Dresden (MBC), Hohe Str. 6, 01069, Dresden, Germany;Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland;The University of Queensland, UQ Diamantina Institute, Translational Research Institute, Brisbane, QLD, Australia;Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia;Griffith Health Institute, Regenerative Medicine Centre, Gold Coast, QLD 4222, Australia

关键词: Matrices;    Tissue engineering;    Vascularisation;    Co-culture;   

Others  :  1131031
DOI  :  10.1186/2045-824X-6-13

 received in 2014-02-16, accepted in 2014-06-12,  发布年份 2014

【 摘 要 】

In vitro pre-vascularization is one of the main vascularization strategies in the tissue engineering field. Culturing cells within a tissue-engineered construct (TEC) prior to implantation provides researchers with a greater degree of control over the fate of the cells. However, balancing the diverse range of different cell culture parameters in vitro is seldom easy and in most cases, especially in highly vascularized tissues, more than one cell type will reside within the cell culture system. Culturing multiple cell types in the same construct presents its own unique challenges and pitfalls. The following review examines endothelial-driven vascularization and evaluates the direct and indirect role other cell types have in vessel and capillary formation. The article then analyses the different parameters researchers can modulate in a co-culture system in order to design optimal tissue-engineered constructs to match desired clinical applications.

【 授权许可】

   
2014 Baldwin et al.; licensee BioMed Central Ltd.

【 图 表 】

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