Bacterial and protozoal agents of canine vector-borne diseases in the blood of domestic and stray dogs from southern Portugal

Particle and Fibre Toxicology

Lenea Campino⁸  Luís Cardoso⁵  Maria Luísa Vieira⁹  Mónica Nunes⁹  Nuno Neves¹  Pedro Silva⁴  Filipe Martinho⁷  Ângela Martins²  Cláudia Ramos⁶  José Manuel Cristóvão⁸  Maria Catarina Fernandes^1,10  Mónica Coimbra³  Bruno Almeida^1,10  Carla Maia^1,10 

[1] Clube Animal, Beja, Portugal;Hospital Veterinário da Arrábida, Azeitão, Portugal;Clínica Veterinária Porto Seguro, Olhão, Portugal;Vetévora, Évora, Portugal;Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal;Unidade de Parasitologia Médica, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), Lisbon, Portugal;Nyctea Lda., Lisbon, Portugal;Global Health and Tropical Medicine, IHMT-UNL, Lisbon, Portugal;Unidade de Microbiologia Médica, IHMT-UNL, Lisbon, Portugal;Faculdade de Medicina Veterinária, Universidade Lusófona de Humanidades e Tecnologias, Lisbon, Portugal

关键词: Polymerase chain reaction;    Portugal;    Protozoa;    Bacteria;    Canine vector-borne diseases;    Dogs;   

Others  :  1146436
DOI  :  10.1186/s13071-015-0759-8

 received in 2015-01-15, accepted in 2015-02-23,  发布年份 2015

【 摘 要 】

Background

The so-called canine vector-borne diseases (CVBD) are caused by a wide range of pathogens transmitted by arthropods. In addition to their veterinary importance, many of these canine vector-borne pathogens can also affect the human population due to their zoonotic potential, a situation that requires a One Health approach. As the prevalence of vector-borne pathogens in cats from southern Portugal has been recently evaluated, the aim of the present study was to assess if the same agents were present in dogs living in the same area, and to assess positivity-associated risk factors.

Methods

One thousand and ten dogs (521 domestic and 489 stray) from veterinary medical centres and animal shelters in southern Portugal were enrolled. Anaplasma spp./Ehrlichia spp., Bartonella spp., Borrelia burgdorferi sensu lato, Babesia spp., Hepatozoon spp. and Leishmania infantum infections were evaluated by polymerase chain reaction (PCR) assays in blood samples.

Results

Sixty-eight (6.7%) dogs were PCR-positive to at least one of the tested CVBD agent species, genera or complex, including one dog found positive to two different genera. Nineteen (1.9%) dogs were positive to Anaplasma spp./Ehrlichia spp., eight (0.8%) to B. burgdorferi s.l., 31 (3.1%) to Hepatozoon spp. and 11 (1.1%) to L. infantum. Anaplasma platys, Ehrlichia canis, B. burgdorferis.l. and Hepatozoon canis were identified by DNA sequencing, including one animal confirmed with both A. platys and H. canis. Furthermore, Wolbachia spp. was amplified in blood from four dogs. None of the tested dogs was positive by PCR for Bartonella spp. or Babesia spp.

Conclusions

The molecular identification of CVBD agents in southern Portugal, some of them with zoonotic concern, reinforces the importance to alert the veterinary community, owners and public health authorities to prevent the risk of transmission of vector-borne pathogens among dogs and to other vertebrate hosts including humans. The prevalence of the selected pathogens was lower than that previously found in cats from the same region, probably because veterinarians and owners are more aware of them in the canine population and control measures are used more often.

【 授权许可】

   
2015 Maia et al.; licensee BioMed Central.

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