MiRNA expression patterns predict survival in glioblastoma

Radiation Oncology

Claus Belka⁵  Simone Mörtl³  Mike Atkinson³  Jörg-Christian Tonn²  Klaus Schulze-Osthoff¹  Hans Kretzschmar⁴  Sabina Eigenbrod⁴  Olivier M Niemöller⁵  Franz Zehentmayr⁵  Maximilian Niyazi⁵ 

[1] Interfakultäres Institut für Biochemie, University of Tübingen, Hoppe-Seyler-Str. 4, 72076 Tübingen, Germany;Department of Neurosurgery, Ludwig-Maximilians-University Munich, Marchioninistr. 15, 81377 Munich, Germany;Helmholtz Zentrum München, Institute of Radiation Biology, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany;Department of Neuropathology, Ludwig-Maximilians-University Munich, Marchioninistr. 15, 81377 Munich, Germany;Department of Radiation Oncology, Ludwig-Maximilians-University Munich, Marchioninistr. 15, 81377 Munich, Germany

关键词: prognosis;    methylation;    microRNA;    glioblastoma;    radiotherapy;   

Others  :  1223820
DOI  :  10.1186/1748-717X-6-153

 received in 2011-07-18, accepted in 2011-11-10,  发布年份 2011

【 摘 要 】

Background

In order to define new prognostic subgroups in patients with glioblastoma a miRNA screen (> 1000 miRNAs) from paraffin tissues followed by a bio-mathematical analysis was performed.

Methods

35 glioblastoma patients treated between 7/2005 - 8/2008 at a single institution with surgery and postoperative radio(chemo)therapy were included in this retrospective analysis. For microarray analysis the febit biochip "Geniom^® Biochip MPEA homo-sapiens" was used. Total RNA was isolated from FFPE tissue sections and 1100 different miRNAs were analyzed.

Results

It was possible to define a distinct miRNA expression pattern allowing for a separation of distinct prognostic subgroups. The defined miRNA pattern was significantly associated with early death versus long-term survival (split at 450 days) (p = 0.01). The pattern and the prognostic power were both independent of the MGMT status.

Conclusions

At present, this is the first dataset defining a prognostic role of miRNA expression patterns in patients with glioblastoma. Having defined such a pattern, a prospective validation of this observation is required.

【 授权许可】

   
2011 Niyazi et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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