Vacuum Seed Sowing Manifold: a novel device for high-throughput sowing of Arabidopsis seeds

Plant Methods

Patrick Masson²  Thomas Rockwell Mackie¹  Benjamin Cox¹  Richard Barker² 

[1] Department of Medical Physics, University of Wisconsin at Madison, 1111 Highland Avenue, Room 1005, Madison, WI 53705, USA;Laboratory of Genetics, Agriculture and life sciences, University of Wisconsin at Madison, 3302 Genetic/Biotech center, 425 C Henry Mall, Madison, WI 53706, USA

关键词: Novel device;    VSSM;    Seed-sowing;    High-throughput;    Arabidopsis;   

Others  :  811249
DOI  :  10.1186/1746-4811-9-41

 received in 2013-06-18, accepted in 2013-08-18,  发布年份 2013

【 摘 要 】

The small size of Arabidopsis provides both opportunities and difficulties for laboratory research. Large numbers of plants can be grown in a relatively small area making it easy to observe and investigate interesting phenotypes. Conversely, their small size can also make it difficult to obtain large quantities of tissue for investigation using modern molecular techniques. Sowing large numbers of their seed can overcome this; however, their small seed size makes this difficult. Here we present the Vacuum Seed Sowing Manifold (VSSM), a simple device that can be printed using a 3D printer and provides a new high throughput method to sow large numbers of seeds at a range of densities.

【 授权许可】

   
2013 Barker et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Arabidopsis Genome Initiative: Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 2000, 408:796-815.
• [2]Parkin IA, Gulden SM, Sharpe AG, Lukens L, Trick M, Olborn TC, Lydiate DJ: Segmental structure of the brassica napus genome based on comparative analysis with Arabidopsis thaliana. Genetics 2005, 171:765-781.
• [3]Cao J, Schneeberger K, Ossowski S, Günther T, Bender S, Fitz J, Koenig D, Lanz C, Stegle O, Lippert C, Wang X, Ott F, Müller J, Alonso-Blanco C, Borgwardt K, Schmid KJ, Weigel D: Whole-genome sequencing of multiple Arabidopsis thaliana populations. Nat Genet 2011, 43:956-963.
• [4]Sessions A, Burke E, Presting G, Aux G, McElver J, Patton D, Dietrich B, Ho P, Bacwaden J, Ko C, Clarke JD, Cotton D, Bullis D, Snell J, Miguel T, Hutchison D, Kimmerly B, Mitzel T, Katagiri F, Glazebrook J, Law M, Goff SA: A high-throughput Arabidopsis reverse genetics system. Plant Cell Online 2002, 14:2985-2994.
• [5]Wang Z, Gerstein M, Snyder M: RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet 2009, 10:57-63.
• [6]Schmidt D, Wilson MD, Spyrou C, Brown GD, Hadfield J, Odom DT: ChIP-seq: using high-throughput sequencing to discover protein–DNA interactions. Methods 2009, 48:240-248.
• [7]Wiese S, Reidegeld KA, Meyer HE, Warscheid B: Protein labeling by iTRAQ: a new tool for quantitative mass spectrometry in proteome research. Proteomics 2007, 7:340-350.
• [8]Kai CC, Fai LK, Chu-Sing L: Rapid prototyping: principles and applications in manufacturing. Singapore: World Scientific Publishing Co., Inc; 2003.
• [9]Kai CC: Three-dimensional rapid prototyping technologies and key development areas. Comput Contr Eng J 1994, 5:200-206.
• [10]Webb P: A review of rapid prototyping (RP) techniques in the medical and biomedical sector. J Med Eng Technol 2000, 24:149-453.