iTRAQ-based quantitative proteomics analysis of rice leaves infected by Rice stripe virus reveals several proteins involved in symptom formation

Virology Journal

Xifeng Wang²  Chuantao Lu¹  Yingdang Ren¹  Jamal-U-Ddin Hajano²  Biao Wang² 

[1] Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China

关键词: Plant defense;    Peptidase;    Magnesium chelatase;    iTRAQ;    Proteome;    Rice;   

Others  :  1224915
DOI  :  10.1186/s12985-015-0328-y

 received in 2015-05-01, accepted in 2015-06-18,  发布年份 2015

【 摘 要 】

Background

Rice plants infected by Rice stripe virus (RSV) usually leads to chlorosis and death of newly emerged leaves. However, the mechanism of RSV-induced these symptoms was not clear.

Methods

We used an iTRAQ approach for a quantitative proteomics comparison of non-infected and infected rice leaves. RT-qPCR and Northern blot analyses were performed for assessing the transcription of candidate genes.

Results

As a whole, 681 (65.8 % downregulated, 34.2 % upregulated infected vs. non-infected) differentially accumulated proteins were identified. A bioinformatics analysis indicated that ten of these regulated proteins are involved in chlorophyll biosynthesis and three in cell death processes. Subsequent RT-qPCR results showed that downregulation of magnesium chelatase was due to reduced expression levels of the genes encoding subunits CHLI and CHLD, which resulted in chlorophyll reduction involved in leaf chlorosis. Three aspartic proteases expressed higher in RSV-infected leaves than those in the control leaves, which were also implicated in RSV-induced cell death. Northern blot analyses of CHLI and p0026h03.19 confirmed the RT-qPCR results.

Conclusions

The magnesium chelatase and aspartic proteases may be associated with RSV-induced leaf chlorosis and cell death, respectively. The findings may yield new insights into mechanisms underlying rice stripe disease symptom formation.

【 授权许可】

   
2015 Wang et al.

【 图 表 】

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