Occurrence and distribution of pepper veinal mottle virus and cucumber mosaic virus in pepper in Ibadan, Nigeria

Virology Journal

Kehinde Titilope Kareem¹  Olusegun Samuel Balogun²  Olawale Arogundade¹ 

[1] National Horticultural Research Institute, Idi Ishin, Jericho Reservation Area, P.M.B 5432, Dugbe, Ibadan, Oyo State, Nigeria;University of Ilorin, Faculty of Agriculture, Crop Protection Department, P.M.B 1515, Ilorin, Kwara State, Nigeria

关键词: Nigeria;    PVMV;    ELISA;    Diagnostic survey;    CMV;    Antisera;   

Others  :  1154793
DOI  :  10.1186/1743-422X-9-79

 received in 2011-07-13, accepted in 2012-04-11,  发布年份 2012

【 摘 要 】

Viral diseases constitute obstacles to pepper production in the world. In Nigeria, pepper plants are primarily affected by pepper veinal mottle virus (PVMV), Cucumber mosaic virus (CMV), Pepper leaf curl Virus (TLCV), Tobacco mosaic virus (TMV), Pepper mottle virus (PMV) and a host of other viruses. The experiment was carried out with a diagnostic survey on the experimental field of the National Horticultural Research Institute, Ibadan, Nigeria and on pepper farms in six local government areas within Ibadan Oyo State, Nigeria, forty samples were collected from each of the farms. Diseased samples were obtained from the field and taken to the laboratory for indexing. In ELISA test some of the samples from the pepper farms showed positive reaction to single infection with PVMV (36.79%), CMV (22.14%) while some others showed positive reaction to mixed infection of the two viruses (10%) but some also negative reaction to PVMV and CMV antisera (31.07).

【 授权许可】

   
2012 Arogundade et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Matthews REF: Plant Virology. 3rd edition. San Diego: Academic; 1991:835.
• [2]Otsuki Y, Takebe I: Double infection of isolated tobacco mesophyll protoplasts by unrelated viruses. J Gen Virol 1976, 30:309-316.
• [3]Vance VB: Replication of potato virus × is altered in co-infections with potato virus Y. Virology 1991, 182:486-489.
• [4]Harrison BD, Finch JT, Gibbs AJ, Hollings M, Shepherd RJ, Valenta V, Wetter C: Sixteen groups of plant viruses. Virology 1971, 45:356-363.
• [5]Lal SB, Singh S: Identification of some virus diseases of vegetable crops in Afghanistan. Plant Prot Bull (Faridabad) 1988, 36(2):83-89.
• [6]Nagaraju R, Reddy HR: Occurrence and distribution of bell pepper viruses around Bangalore. Volume 10. Current research, University of Agricultural Sciences, Bangalore; 1980::155; 6.
• [7]Huguenot C, Furneaux MT, Clare J, Hamilton RI: Sero-diagnosis of Pepper veinal mottle viru in West Africa using specific monoclonal antibodies in DAS-ELISA. J Phytopathol 1996, 144:29-32.
• [8]Alegbejo MD, Uvah II: Effect of intercropping pepper with tall companion crops on the incidence of Pepper veinal mottle viru on pepper. Nigerian J Entomology 1987, 7:82-87.
• [9]Fajinmi AA: The incidence, spread and possible control strategies of Pepper veinal mottle Potyvirus (PVMV) disease on pepper (Capsicum annuum.L.) in Nigeria. In Ph. D thesis. University of Ibadan Oyo State Nigeria; 2006.
• [10]Atiri GI: A disease of fluted pumpkin (Telfairia occidentalis Hook.f.) caused by a yellow vein clearing strain of Pepper veinal mottle virus in Nigeria. J Plant Prot Tropics 1986, 3(2):105-110.
• [11]Brunt AA, Kenten RH, Phillips S: Symptomatologically distinct strains of Pepper veinal mottle viru from four (4). West African Solanaceous crops. Ann App Biology 1978, 88:115-119.
• [12]Igwegbe ECK, Waterworth HE: Properties and serology of the strain of Pepper veinal mottle virus isolated from eggplant (Solanum melongena L.) in Nigeria. Phytopathologische Zeitschrift 1982, 103(1):9-12.
• [13]Ladipo JL, Roberts IM: Pepper veinal mottle viru associated with a streak disease of tomato in Nigeria. Ann Appl Biol 1977, 87:133-138.
• [14]Prasada Rao, RDVJ Yaraguntaiah RC: The occurrence of Pepper veinal mottle virus on chilli in India. Mysore J Agric Sci 1979, 13(4):445-448; 6.
• [15]Crescenzy A: Cucumber mosaic cucumovirus populations in Italy under natural epidemic conditions and after a satellite - mediated protection test. Plant Dis 1993, 77:28-33.
• [16]Palukaitis P, Roossinck MJ, Dietzgen RG, Francki RIB: Cucumber mosaicvirus. Adv Virus Res. 1992, 41:281-349.
• [17]Alegbejo MD: Identification of a weed host of Pepper veinal mottle virus in Northern Nigeria. Samaru J Agric Res 1987, 5(1 and 2):65-70.
• [18]Atiri GI: Progress of Pepper veinal mottle viru disease in Capsicu peppers. Crop Prot 1992, 11(3):255-259.
• [19]Arogundade O, Balogun OS, Shokalu O, Aliyu TH: Influence of Cowpea Mottle Virus and Cucumber Mosaic Virus on the Growth and Yield of six Lines of Soybean (Glycine max L.). J Agric Sci 2010, 2(1):72-78.