Placental lipase expression in pregnancies complicated by preeclampsia: a case–control study

Reproductive Biology and Endocrinology

Marloes Dekker Nitert¹  Leonie K. Callaway¹  H. David McIntyre²  Trent M. Woodruff³  Kerina J. Denny³  Katherin Scholz Romero⁴  Marta H. Kubala⁴  Helen L. Barrett¹ 

[1] School of Medicine, The University of Queensland, Herston, QLD, Australia;Mater Research Institute, The University of Queensland Brisbane, Brisbane, QLD, Australia;School of Biomedical Sciences, The University of Queensland, St Lucia, QLD, Australia;UQ Centre for Clinical Research, The University of Queensland, Herston, QLD, Australia

关键词: Pregnancy;    Placenta;    Lipase;    Intrauterine growth restriction;    Preeclampsia;   

Others  :  1224966
DOI  :  10.1186/s12958-015-0098-9

 received in 2015-06-23, accepted in 2015-08-20,  发布年份 2015

【 摘 要 】

Background

Preeclampsia (PE) is associated with maternal and neonatal morbidity and mortality. In PE, the physiological hyperlipidaemia of pregnancy is exaggerated. The purpose of this study was to examine the expression of adipose triglyceride lipase (ATGL), hormone sensitive lipase (HSL), lipoprotein lipase (LPL) and endothelial lipase (EL) in pregnancies complicated by PE.

Methods

Placentae were collected from 16 women with PE and 20 women with uncomplicated pregnancies matched for maternal prepregnancy BMI and gestational age of delivery. Gene and protein expression of the placental lipases were measured by Q-PCR and Western blot. DNA methylation of the promoter of LPL was assessed by bisulfite sequencing. Lipase localisation and activity were analysed.

Results

Gene expression of all lipases was significantly reduced, as was HSL protein level in women with PE. All lipases were localised to trophoblasts and endothelial cells in PE and control placentae. There was no difference in methylation of the LPL promoter between PE and control placentae. Lipase activity was not altered in placentae from women with PE.

Conclusion

These results suggest that the decreased placental lipase gene but not protein expression or lipase activity, which is associated with late-onset PE is not a major contributor to the abnormal lipids seen in PE.

【 授权许可】

   
2015 Barrett et al.

【 图 表 】

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