Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

Plant Methods

Michael R Blatt¹  Adrian Hills¹  Xin-Qin Xu¹  Cornelia Eisenach¹  Zhong-Hua Chen² 

[1] Laboratory of Plant Physiology and Biophysics, Institute of Molecular, Cell and Systems Biology, University of Glasgow, Glasgow, G12 8QQ, UK;School of Science and Health, University of Western Sydney, Richmond, NSW 2753, Australia

关键词: Arabidopsis;    Mutant analysis;    Membrane conductance;    Cl^- channel, Voltage-gated;    K⁺ channel (voltage-gated);    Microelectrode;   

Others  :  822540
DOI  :  10.1186/1746-4811-8-15

 received in 2011-08-13, accepted in 2012-04-10,  发布年份 2012

【 摘 要 】

Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K⁺ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.

【 授权许可】

   
2012 Chen et al.; licensee BioMed Central Ltd.

【 图 表 】

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