Comparison of the biopsy and cytobrush techniques for diagnosis of subclinical endometritis in mares

Reproductive Biology and Endocrinology

Marta J Siemieniuch¹  Zdzisław Staroniewicz³  Andrzej Raś²  Marcin Nowak³  Roland Kozdrowski³  Justyna Buczkowska³ 

[1] Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland;Department and Clinic of Reproduction, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland;Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland

关键词: Cytology;    Cytobrush;    Biopsy;    Endometritis;    Mare;   

Others  :  804445
DOI  :  10.1186/1477-7827-12-27

 received in 2014-01-15, accepted in 2014-03-31,  发布年份 2014

【 摘 要 】

Background

Endometritis is a major cause of infertility in the mare. Therefore, the diagnosis of this disease is very important in veterinary practice. The objective of this study was to compare bacteriological and cytological results obtained from the mare uterus using biopsy (EB) and cytobrush (CB) techniques and relating these findings to the presence of polymorphonuclear cells (PMNs) in endometrial tissue as the gold standard for detection of endometritis. In particular, we tested the hypothesis that endometrial cytology and microbiology data obtained from material collected using the EB and CB techniques are similar, so that the CB technique could preferentially be used to detect subclinical endometritis in clinical practice.

Methods

A total of 69 mares suspected of subclinical endometritis because of previous reproductive history and 15 maiden mares were enrolled in this study. Material collected from both EB and CB was smeared on sterile glass slides for cytological examinations and on culture media for microbiological examinations. Bacteriological cultures and cytological samples were classified as negative (no growth or mixed cultures of more than three microorganisms; <2% PMNs) or positive (pure growth of microorganisms; >2% PMNs) for endometritis.

Results

Positive growth was observed in 43% of CB samples and in 54% of EB samples (difference not significant). The growth of β-hemolytic streptococci was always connected with positive cytology. This relationship was not observed for growth of E. coli or for non-pathogenic flora. The sensitivity of bacterial growth and cytology from EB was 0.63 and 0.73 respectively. The sensitivities of bacterial growth and cytology from CB were 0.50 and 0.71 respectively.

Conclusion

Microbiological and cytological results obtained from CB are similar to those obtained from EB and based on these findings the CB technique may be recommended for collection of materials from the mare’s uterus in clinical practice.

【 授权许可】

   
2014 Buczkowska et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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