Reproductive Biology and Endocrinology | |
MicroRNA-181a is involved in the regulation of human endometrial stromal cell decidualization by inhibiting Krüppel-like factor 12 | |
Yali Hu1  Guijun Yan1  Haixiang Sun1  Xin Zhen1  Lijun Ding1  Zhenyu Diao1  Bai Xue2  Yue Jiang1  Hui Zhang2  Qun Zhang1  | |
[1] Reproductive Medicine Center, Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, Jiangsu, China;Reproductive Medicine Center, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing 210029, Jiangsu, China | |
关键词: Decidualization; Human endometrial stromal cell; KLF12; MicroRNA-181a; | |
Others : 1157033 DOI : 10.1186/s12958-015-0019-y |
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received in 2014-11-11, accepted in 2015-03-14, 发布年份 2015 | |
【 摘 要 】
Background
The transformation of endometrium into decidua is essential for normal implantation of the blastocyst. However, the post-transcriptional regulation and the miRNAs involved in decidualization remain poorly understood. Here, we examined microRNA-181a (miR-181a) expression in decidualized human endometrial stromal cell (hESC). In addition, we investigated the functional effect of miR-181a on hESC decidualization in vitro.
Methods
Quantitative real-time PCR (qRT-PCR) was used to detect the profile of miR-181a in decidualized hESC. qRT-PCR, enzyme-linked fluorescent assay, and immunofluorescence assay were performed to investigate decidualization marker genes’ expression after enhancing or inhibition of miR-181a expression in hESC. Luciferase reporter assay, western blotting, qRT-PCR, and immunofluorescence assay were carried out to identify the relationship between miR-181a and Krüppel-like factor 12 (KLF12).
Results
miR-181a expression levels increased dramatically in hESC treated with 8-Br-cAMP and MPA. Increased miR-181a expression promoted hESC decidualization-related gene expression and morphological transformation; conversely, inhibition of miR-181a expression compromised hESC decidualization in vitro. Further analysis confirmed that miR-181a interacted with the 3′ untranslated region of the transcription factor KLF12 and down-regulated KLF12 at the transcriptional and translational levels. KLF12 overexpression abolished miR-181a-induced decidualization.
Conclusions
Our findings suggest that miR-181a plays a functionally important role in human endometrial stromal cell decidualization in vitro by inhibiting KLF12.
【 授权许可】
2015 Zhang et al.; licensee BioMed Central.
【 预 览 】
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