Adipose-derived stem cells improved mouse ovary function after chemotherapy-induced ovary failure

Stem Cell Research & Therapy

Yuanqing Yao⁵  Changyong Wang³  Fang Gu⁵  Ling Yu¹  Yi Li²  Shufang Wang⁴  Min Sun² 

[1] Department of Obstetrics and Gynecology, First Affiliated Hospital of the General Hospital of the People’s Liberation Army, Beijing, P.R. China;Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi’an, P.R. China;Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, P.R. China;Department of Blood Transfusion, The General Hospital of the People’s Liberation Army, Beijing, P.R. China;Department of Obstetrics and Gynecology, The General Hospital of the People’s Liberation Army, No. 28, Fuxing Road, Beijing 100875, P.R. China

关键词: ADSC transplantation;    Reproduction;    Stem cell therapy;    Ovary;    ADSC;    CTX;    POF;   

Others  :  847317
DOI  :  10.1186/scrt231

 received in 2013-03-12, accepted in 2013-07-03,  发布年份 2013

【 摘 要 】

Introduction

Young patients receiving chemotherapy occasionally face infertility and premature ovarian failure (POF). Numerous investigations reported that adipose-derived stem cells (ADSCs) transplantation could ameliorate the structure and function of injured tissues. The aim of this study was to explore the therapeutic efficacy of ADSC transplantation for chemotherapy-induced ovarian damage.

Methods

Female mice were injected intraperitoneally with 50 mg/kg cyclophosphamide (CTX). After 15 consecutive days of injection, ADSCs were transplanted either directly into bilateral ovaries or via intravenous injection, and the ovaries were excised after either 1 week or 1 month of treatment. The follicles were counted and categorized, and ovarian histologic sections were stained for TUNEL. Ovarian function was evaluated by monitoring ovulation. ADSC tracking, microarray analyses, and real-time polymerase chain reaction (PCR) were used to assess the inner mechanism of injury and repair.

Results

The ovarian function of mice exposed to CTX injection improved after ADSC transplantation. The population of follicles at different stages and ovulation significantly increased after the treatment. Immunofluorescence revealed reduced TUNEL staining. The tracking of ADSCs revealed that these cells did not directly differentiate into the follicle component. Microarray analyses indicated that changes in different groups of genes might affect follicle formation or ovulation.

Conclusions

ADSC transplantation improved ovarian function. Our results suggest a potential mechanism for ADSC therapy.

【 授权许可】

   
2013 Sun et al.; licensee BioMed Central Ltd.

【 图 表 】

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