Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay

Virology Journal

Liu Zuo-Hua¹  Xiong Tao²  Peng Xiang-Wei¹  Shen Ke-Fei¹  Yang Rui¹  Yang Jin-Long¹ 

[1] Chongqing Academy of Animal Science, Chongqing 402460, China;Rongchang Bureau of Animal Husbandry, Chongqing 402460, China

关键词: Honeybee;    SYBR Green;    Loop-mediated isothermal amplification;    Sacbrood virus;   

Others  :  1155026
DOI  :  10.1186/1743-422X-9-47

 received in 2011-06-10, accepted in 2012-02-17,  发布年份 2012

【 摘 要 】

Background

Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required.

Findings

A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green.

Conclusions

The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity.

【 授权许可】

   
2012 Jin-Long et al; licensee BioMed Central Ltd.

【 图 表 】

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