Transcriptome analysis of anti-fatty liver action by Campari tomato using a zebrafish diet-induced obesity model

Nutrition & Metabolism

Toshio Tanaka⁴  Norihiro Nishimura²  Yuhei Nishimura⁴  Takehiko Oka³  Liqing Zang²  Junya Kuroyanagi³  Yasuhito Shimada⁴  Toshiyuki Tainaka¹ 

[1] Delica Foods Co Ltd, Tokyo, Japan;Department of Translational Medical Science, Mie University Graduate School of Medicine, Mie, Japan;Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Mie, Japan;Department of Omics Medicine, Mie University Industrial Technology Innovation Institute, Mie, Japan

关键词: DNA microarray;    zebrafish;    diet-induced obesity;    vegetables;    liver steatosis;    dyslipidemia;   

Others  :  820204
DOI  :  10.1186/1743-7075-8-88

 received in 2011-08-22, accepted in 2011-12-13,  发布年份 2011

【 摘 要 】

Background

High dietary intake of vegetable products is beneficial against obesity and its related diseases such as dyslipidemia, nonalcoholic fatty liver disease, and cancer. We previously developed a diet-induced obesity model of zebrafish (DIO-zebrafish) that develops visceral adiposity, dyslipidemia, and liver steatosis. Zebrafish is a polyphagous animal; thus we hypothesized that DIO-zebrafish could be used for transcriptome analysis of anti-obesity effects of vegetables.

Results

Each vegetable exhibited different effects against obesity. We focused on "Campari" tomato, which suppressed increase of body weight, plasma TG, and lipid droplets in livers of DIO-zebrafish. Campari tomato decreased srebf1 mRNA by increase of foxo1 gene expression, which may depend on high contents of β-carotene in this strain.

Conclusions

Campari tomato ameliorates diet-induced obesity, especially dyslipidemia and liver steatosis via downregulation of gene expression related to lipogenesis. DIO-zebrafish can discriminate the anti-obesity effects of different strains of vegetables, and will become a powerful tool to assess outcomes and find novel mechanisms of anti-obesity effects of natural products.

【 授权许可】

   
2011 Tainaka et al; licensee BioMed Central Ltd.

【 图 表 】

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