Avian leukosis virus subgroup J associated with the outbreak of erythroblastosis in chickens in China

Virology Journal

Ziqiang Cheng¹  Xiaomin Zhao¹  Yue Wang²  Linin Yu²  Yanping Jiang²  Guihua Wang¹ 

[1] Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, China;Department of Fundamental Veterinary, Molecular pathology lab, College of Veterinary Medicine, Shandong Agricultural University, Tai’an, China

关键词: Immunohistochemistry;    PCR;    Avian leukosis virus subgroup J;    Erythroblastosis;   

Others  :  1151231
DOI  :  10.1186/1743-422X-10-92

 received in 2012-11-02, accepted in 2013-03-11,  发布年份 2013

【 摘 要 】

Background

Emaciation, depression and lethargy were observed in two flocks of Chinese local breed and one flock of commercial layer chicken infected naturally from 2010 to 2011. The aims of this study were to diagnose.

Methods and results

Gross observation showed that severe enlargement of liver, spleen and kidney, and hemorrhage of thymus, muscle and glandular stomach in all submitted birds. The liver and lung of one flock had diffuse, multifocal white raised foci on the surface as well as on the cut-surface. Numerous erythrocytoblasts with bigger volume, basophilic cytoplasm and round nucleus were observed in blood and bone marrow smears. The same erythrocytoblasts were also found crowded in blood vessels and mesenchym of tissues by histological examination, and some had mitotic figures. PCR results showed that three flocks were positive for ALV-J with specific fragment of 924 bp, negative for AEV, ALV-A, ALV-B, Marek’s disease virus (MDV) and Reticuloendotheliosis virus (REV). The results of immunohistochemistry showed that cytoplasm of histiocytes and erythrocytoblasts in lung and spleen sections was positive for ALV-J antigen.

Conclusion

These data demonstrated that erythroblastosis was all induced by ALV-J in the three different flocks. This is the first document report of erythroblastosis induced by ALV-J in China flocks.

【 授权许可】

   
2013 Wang et al.; licensee BioMed Central Ltd.

【 图 表 】

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