Interaction of milk proteins and Binder of Sperm (BSP) proteins from boar, stallion and ram semen

Reproductive Biology and Endocrinology

Puttaswamy Manjunath¹  Michel Lafleur²  Marie-France Lusignan³  Geneviève Plante³ 

[1] Puttaswamy Manjunath, Maisonneuve-Rosemont Hospital Research Center, 5415 L’Assomption blvd, MontréalH1T 2M4Québec, Canada;Department of Chemistry, Center for Self-Assembled Chemical Structures, Université de Montréal, C.P. 6128, Succ. Centre Ville, MontréalH3C 3J7Québec, Canada;Maisonneuve-Rosemont Hospital Research Center, 5415 L’Assomption blvd, MontréalH1T 2M4Québec, Canada

关键词: BSP proteins;    Protein-protein interaction;    Whey proteins;    Caseins;    Milk extender;    Sperm preservation;   

Others  :  1224973
DOI  :  10.1186/s12958-015-0093-1

 received in 2015-05-31, accepted in 2015-08-06,  发布年份 2015

【 摘 要 】

Background

Mammalian semen contains a family of closely related proteins known as Binder of SPerm (BSP proteins) that are added to sperm at ejaculation. BSP proteins extract lipids from the sperm membrane thereby extensively modifying its composition. These changes can ultimately be detrimental to sperm storage. We have demonstrated that bovine BSP proteins interact with major milk proteins and proposed that this interaction could be the basis of sperm protection by milk extenders. In the present study, we investigated if homologous BSP proteins present in boar, stallion and ram seminal plasma display a similar affinity for the milk proteins in order to assess whether the mechanism of sperm protection by milk for these species could be general.

Methods

Skim milk was incubated with seminal plasma proteins (boar, stallion and ram), chromatographed on a Sepharose CL-4B column and protein fractions were analyzed by immunoblotting.

Results

Boar, stallion and ram BSP proteins displayed affinity for a milk protein fraction (F1) mainly composed of α-lactalbumin, β-lactoglobulin, and κ-casein. They also had affinity for another milk protein fraction (F2) composed mostly of casein micelles. However, stallion BSP showed higher affinity for the fraction (F1).

Conclusions

These results further extend our view that the association of BSP proteins with milk proteins could be a general feature of the mechanism of mammalian sperm protection by milk to prevent detrimental effect of prolonged exposure of sperm to seminal plasma.

【 授权许可】

   
2015 Plante et al.

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