Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatment

Particle and Fibre Toxicology

Yinong Duan⁴  Yongwei Qin⁴  Lingbo Zhang⁴  Xijuan Gu¹  Hongyan Qian³  Xiaolei Sun⁴  Jianxin Wang²  Jinling Chen⁴  Ke Song⁴  Dandan Zhu⁴ 

[1] Nantong University Xinglin College, Nantong 226001, Jiangsu, People’s Republic of China;Laboratory Medicine Center, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, People’s Republic of China;Cancer Research Center, Affiliated Tumor Hospital of Nantong University, Nantong 226001, Jiangsu, People’s Republic of China;Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People’s Republic of China

关键词: Liver fibrosis;    Inflammation;    SEPT4;    Schistosoma japonicum;   

Others  :  1147907
DOI  :  10.1186/s13071-015-0640-9

 received in 2014-08-27, accepted in 2015-01-03,  发布年份 2015

【 摘 要 】

Background

Septin4 (SEPT4) exists widely in human tissues and is related to mechanical stability, actin dynamics, membrane trafficking, viral replication and apoptosis. Data from many studies have suggested that SEPT4 plays a significant role in liver fibrosis. SEPT4 is down-regulated in the model of CCl₄ and BDL treated liver fibrosis. However, it is up-regulated and peaked at 12 weeks post-infection (p.i.), and then decreased subsequently in Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. The aim of this study was to observe the dynamic alteration of SEPT4 after the treatment of praziquantel (PZQ) in ICR mice infected with S. japonicum.

Methods

Expression of SEPT4 was determined by western blot, immunofluorescence and qRT-PCR. And pro-inflammatory cytokines IL-6 and TNF-α were detected by qRT-PCR. The number of eggs, the diameter of egg granulomas and fibrosis-associated genes were also measured.

Results

Our results showed that the granulomatous inflammation was reduced, whereafter the expression of SEPT4 on hepatic stellate cells (HSCs) was decreased after PZQ anti-schistosome therapy. And the variation tendency of SEPT4 had positive correlation with the inflammatory response in the area of S. japonicum egg granulomas.

Conclusions

Based on these findings, the inhibition of the expression of the SEPT4 by PZQ might be due to alleviation of the inflammatory response at the chronic and advanced stage of S. japonicum infection.

【 授权许可】

   
2015 Zhu et al.; licensee BioMed Central.

【 图 表 】

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