Blue-native PAGE in plants: a tool in analysis of protein-protein interactions

Plant Methods

A Harvey Millar²  Hans-Peter Braun¹  Holger Eubel² 

[1] Abteilung Angewandte Genetik, Universität Hannover, Herrenhäuser Str. 2, 30419 Hannover, Germany;ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Hwy, Crawley 6009, Perth, Australia

关键词: solubilization;    Coomassie;    hydrophobic proteins;    protein complexes;    2D-PAGE;    Gel-based Proteomics;   

Others  :  823152
DOI  :  10.1186/1746-4811-1-11

 received in 2005-09-05, accepted in 2005-11-16,  发布年份 2005

【 摘 要 】

Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science.

【 授权许可】

   
2005 Eubel et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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