Improved methodology for assaying brassinosteroids in plant tissues using magnetic hydrophilic material for both extraction and derivatization

Plant Methods

Yu-Qi Feng²  Bi-Feng Yuan²  Jiu-Feng Liu²  Jian-Hong Wu³  Jun Ding¹ 

[1] Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, China;Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China;College of Chemical Engineering, Wuhan Textile University, Wuhan 430200, China

关键词: Liquid chromatography-mass spectrometry;    Hydrophilic interaction;    In situ derivatization;    Magnetic solid phase extraction;    Brassinosteroid;   

Others  :  1132159
DOI  :  10.1186/1746-4811-10-39

 received in 2014-07-31, accepted in 2014-10-30,  发布年份 2014

【 摘 要 】

Background

Brassinosteriods (BRs) are a group of important phytohormones that have major effects on plant growth and development. To fully elucidate the function of BRs, a sensitive BR assay is required. However, most of the previously reported methods are tedious and time-consuming due to multiple pretreatment steps. Therefore, it is of great significance to develop a method to increase the throughput and detection sensitivity of BR analysis.

Results

We established a novel analytical method of BRs based on magnetic solid phase extraction (MSPE) combined with in situ derivatization (ISD). TiO₂-coated magnetic hollow mesoporous silica spere(TiO₂/MHMSS) was served as a double identity- a microextraction sorbent and “microreactor” for the capture and derivatization of BRs in sequence. BRs were first extracted onto TiO₂/MHMSS through hydrophilic interaction. The BR-adsorbed TiO₂/MHMSS was then employed as a “microreactor” for the derivatization of BRs with 4-(N,N-dimethyamino)phenylboronic acid (DMAPBA). The MSPE-ISD method was simple and fast, which could be accomplished within 10 min. Furthermore, the derivatives of BRs showed better MS response because they were incorporated with tertiary amino groups. Uniquely, endogenous BRs were detected in only 100 mg fresh weight plant tissue.

Conclusion

Our proposed MSPE-ISD method for the determination of endogenous BRs is rapid and sensitive. It can be applied to the analysis of endogenous BRs in 100 mg fresh plant tissue (Brassica napus L. (B. napus L)). The proposed strategy for plant sample preparation may be extended to develop analytical methods for determination of a wide range of analytes with poor MS response in other complex sample matrices.

【 授权许可】

   
2014 Ding et al.; licensee BioMed Central Ltd.

【 图 表 】

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