Conformational templating of α-synuclein aggregates in neuronal-glial cultures

Molecular Neurodegeneration

Todd E Golde¹  Benoit I Giasson¹  Jada Lewis¹  Awilda M Rosario¹  Pedro E Cruz¹  Carolina Ceballos-Diaz¹  Michael A Thomas¹  Amanda N Sacino¹ 

[1] Department of Neuroscience, McKnight Brain Institute, Center for Translational Research in Neurodegenerative Disease, University of Florida College of Medicine, 1275 Center Drive, Gainesville, FL 32610, USA

关键词: Prion;    Amyloid;    Self-templating;    Parkinson’s disease;    α-Synuclein;   

Others  :  862330
DOI  :  10.1186/1750-1326-8-17

 received in 2013-02-09, accepted in 2013-05-15,  发布年份 2013

【 摘 要 】

Background

Genetic studies have established a causative role for α-synuclein (αS) in Parkinson’s disease (PD), and the presence of αS aggregates in the form of Lewy body (LB) and Lewy neurite (LN) protein inclusions are defining pathological features of PD. Recent data has established that extracellular αS aggregates can induce intracellular αS pathologies supporting the hypothesis that αS pathology can spread via a “prion-like” self-templating mechanism.

Results

Here we investigated the potential for conformational templating of αS intracellular aggregates by seeding using recombinant wild-type and PD-linked mutant (A53T and E46K) αS in primary mixed neuronal-glial cultures. We find that wild-type and A53T αS fibrils predominantly seed flame-like inclusions in both neurons and astrocytes of mixed primary cultures; whereas the structurally distinct E46K fibrils seed punctate, rounded inclusions. Notably, these differences in seeded inclusion formation in these cultures reflect differences in inclusion pathology seen in transgenic mice expressing the A53T or E46K αS mutants. We further show that the inclusion morphology is dictated primarily by the seed applied rather than the form of αS expressed. We also provide initial evidence that αS inclusion pathology can be passaged in primary astrocyte cultures.

Conclusion

These studies establish for the first time that αS aggregation in cultured cells can occur by a morphological self-templating mechanism.

【 授权许可】

   
2013 Sacino et al.; licensee BioMed Central Ltd.

【 图 表 】

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