A seroepidemiological survey of Crimean Congo hemorrhagic fever among Cattle in North Kordufan State, Sudan

Virology Journal

Imadeldin E Aradaib¹  Mubarak AM Mahmoud¹  Ibrahim A Adam¹ 

[1] Department of Clinical Medicine, Molecular Biology Laboratory (MBL), Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32, Khartoum North, Sudan

关键词: Sudan;    ELISA;    CCHFV;    Viral hemorrhagic fevers;    Survey;    Epidemiology;   

Others  :  1149897
DOI  :  10.1186/1743-422X-10-178

 received in 2013-01-17, accepted in 2013-05-29,  发布年份 2013

【 摘 要 】

Background

Crimean Congo hemorrhagic fever (CCHF), caused by CCHF virus (CCFV), may cause a fatal hemorrhagic illness in humans with mortality rate of approximately 30%. However, in animals the disease is typically asymptomatic and no clinical hemorrhagic infections appears to be associated with CCHFV. Recently, CCHF activity has been detected in western and southern Kordufan region, Sudan. Currently, no information is available in regard to previous exposure of livestock to CCHFV infection in the region.

Aims

In the present study, a seroepidemiological survey was conducted to determine the prevalence of CCHF and to identify the potential risk factors associated with the disease among cattle in North Kordufan State, Sudan.

Methods

In this survey, 299 blood samples were collected randomly from six localities in North Kordufan State and were tested by enzyme-linked immunosorbent assay (ELISA) for detection of CCHFV-specific immunoglobulin G (IgG) antibodies.

Results

The result of the study indicated that the prevalence rate of CCHF was relatively high among cattle, where serological evidence of the infection was observed in 21 (7.0%) of 299 animals. Older cattle were eight times more likely to be infected with the virus (OR=8.0824, CI=1.174-66.317, p-value=0.034). Cross breeds were at 37 time higher at risk compared to endogenous breed (OR=37.06, CI=1.455-944, p-value=0.029). Highly tick-infested cattle are 6 times higher at risk for CCHF when compared to tick-free animals (OR=6.532, CI=1.042-10.852, p-value=0.030).

Conclusion

It is recommended that surveillance of CCHF should be extended to include other ruminant animals and to study the distribution of ticks in the region to better predict and respond to CCHF outbreak in the State of North Kordufan, Sudan.

【 授权许可】

   
2013 Adam et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Swanepoel R, Shepherd AJ, Leman PA: Epidemiological and clinical features of Crimean-Congo hemorrhagic fever in Southern Africa. Am J Trop Med Hyg 1987, 36:120-132.
• [2]Vesenjak-Hirjan J, Punda-Polic V, Dobe M: Geographical distribution of arboviruses in Yugoslavia. J Hyg Epidemiol Microbiol Immunol 1991, 35:129-140.
• [3]Schwarz TF, Nitschko H, Jager G, Nsanze G, Longson G, Pugh RN, Abraham AK: Crimean-Congo haemorrhagic fever in Oman. Lancet 1995, 346:230.
• [4]Schwarz TF, Nsanze H, Longson M, Nitschko M, Gilch S, Shurie S, Ameen A, Zahir AR, Acharya UG, Jager G: Polymerase chain reaction for diagnosis and identification of distinct variants of Crimean-Congo hemorrhagic fever virus in the United Arab Emirates. Am J Trop Med Hyg 1996, 55:190-196.
• [5]Rodriguez LL, Maupin GO, Ksiazek TG, Rollin PE, Khan AS, Schwarz TE, Lofts RF, Smith JF, Noor AM, Peters CJ, Nichol ST: Molecular investigation of a multisource outbreak of Crimean-Congo hemorrhagic fever in the United Arab Emirates. Am J Trop Med Hyg 1997, 57:512-518.
• [6]Altaf A, Luby S, Ahmed AJ, Zaidi N, Khan AJ, Mirza S, Mc Cormick J, Fisher-Hoch S: Outbreak of Crimean-Congo haemorrhagic fever in Quetta, Pakistan: contact tracing and risk assessment. Trop Med Int Health 1998, 1998(3):878-882.
• [7]Burt FJ, Leman PA, Smith JF, Swanepoel R: The use of a reverse transcription-polymerase chain reaction for the detection of viral nucleic acid in the diagnosis of Crimean–Congo haemorrhagic fever. J Virol Methods 1998, 70:129-137.
• [8]Ergonul O: Crimean-Congo haemorrhagic fever. Lancet Infect Dis 2006, 6:203-214.
• [9]Whitehouse CA: Crimean-Congo hemorrhagic fever. Antiviral Res 2004, 64:145-160.
• [10]Avsic-Zupanc T: Epidemiology of Crimean-Congo hemorrhagic fever in the Balkans. In Crimean-Congo Hemorrhagic Fever: A Global Perspective. Edited by Ergonul OWCA. Dordrecht: Springer; 2007:75-88.
• [11]Aradaib IE, Erickson BR, Mustafa ME, Khristova ML, Saeed NS, Elageb RM, Nichol ST: Nosocomial outbreak of Crimean-Congo hemorrhagic fever, Sudan. Emerging Infect Dis 2010, 16:837-839.
• [12]Aradaib IE, Erickson BR, Karsany ME, Khristova ML, Elageb RM, Mohamed MEH, Nichol ST: Multiple Crimean-Congo hemorrhagic fever virus strains are associated with disease outbreaks in Sudan, 2008–2009. PLoS Negl Trop Dis 2011, 5:e1159.
• [13]Elata AT, Karsany MS, Elageb RM, Hussain MA, Eltom KH, Elbashir MI, Aradaib IE: A nosocomial transmission of crimean-congo hemorrhagic fever to an attending physician in north kordufan, Sudan. Virol J 2011, 8:303. http://www.virologyj.com/content/8/1/303 webcite BioMed Central Full Text
• [14]Watts DM, Tigani AE, Botros BA, Salib AW, Olson JG, McCarthy M: Arthropod-borne viral infections associated with a fever outbreak in the northern province of Sudan. J Trop Med Hyg 1994, 97:228-230.
• [15]McCarthy MC, Haberberger RL, Salib AW, Soliman BA, Tigani AE, Khalid OI: Evaluation of arthropod-borne viruses and other infectious disease pathogens as the causes of febrile illnesses in the Khartoum Province of Sudan. J Med Virol 1996, 48:141-146.
• [16]Morrill JC, Soliman AK, Imam IZ, Botros BA, Moussa MI, Watts DM: Serological evidence of Crimean-Congo haemorrhagic fever viral infection among camels imported into Egypt. J Trop Med Hyg 1990, 93:201-204.
• [17]Hassanein KM, El-Azazy A, Yousef HM: Detection of Crimean-Congo haemorrhagic fever virus antibodies in humans and imported livestock in Saudi Arabia. Trans R Soc Trop Med Hyg 1997, 91:536-537.
• [18]Burt FJ, Swanepool R, Braack LEG: Enzyme-linked immunosorbent assays for the detection of antibody to Crimean-Congo haemorrhagic fever virus in the sera of livestock and wild vertebrates. Epidemiol Infect 1993, 111:547-557. 547
• [19]Shepherd AJ, Swanepoel R, Shepherd SP, McGillivray GM, Searle LA: Antibody to Crimean-Congo hemorrhagic fever virus in wild mammals from Southern Africa. Am J Trop Med Hyg 1987, 36:133-142.
• [20]Garcia S, Chinikar S, Coudrier D, Billecocq A, Hooshmand B, Crance JM, Garin D, Bouloy M: Evaluation of a Crimean-Congo hemorrhagic fever virus recombinant antigen expressed by Semliki Forest suicide virus for IgM and IgG antibody detection in human and animal sera collected in Iran. J Clin Virol 2006, 35:154-159.
• [21]Martin SW, Meek AH, Willeberg P: Veterinary epidemiology: principles and methods. Ames, lowa, USA: Iowa State University Press; 1987:P 45.
• [22]Dowall SD, Richards KS, Graham VA, Chamberlain J, Hewson R: Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen. J Virol Methods 2012, 2:335-341.
• [23]Mohamed M, Said A, Murad M, Grahard R: A surological survey of Crimean Congo hemorrhagic fever in animal in the Sharkia Governorate in Egypt. Vet Ital 2008, 44:513-517.
• [24]Swanepoel R, Struthers JK, Shepherd AJ, McGillivray GM, Nel MJ, Jupp PG: Crimean-Congo hemorrhagic fever in South Africa. Am J Trop Med Hyg 1983, 32:1407-1415.
• [25]Swanepoel R, Shepherd AJ, Leman PA, Shepherd SP, Miller GB: A common-source outbreak of Crimean-Congo haemorrhagic fever on a dairy farm. S Afr Med J 1985, 68:635-637.
• [26]Shepherd AJ, Swanepoel R, Leman PA: Antibody response in Crimean-Congo hemorrhagic fever. Rev Infect Dis 1989, 11:801-806.
• [27]Burney MI, Ghafoor A, Saleen M, Webb PA, Casals J: Nosocomial outbreak of viral hemorrhagic fever caused by Crimean hemorrhagic fever-Congo virus in Pakistan in 1976. Am J Trop Med Hyg 1980, 29:941-947.
• [28]Ahmeti S, Raka L: Crimean-Congo haemorrhagic fever in Kosova: A fatal case report. Virol J 2006, 3:85. BioMed Central Full Text
• [29]Gurbuz Y, Sencan I, Ozturk B, Tutuncu E: A case of nosocomial transmission of Crimean-Congo hemorrhagic fever from patient to patient. Int J Infect Dis 2009, 13:105-107.
• [30]Khan AS, Maupin GO, Rollin PE, Noor AM, Shurie HH, Shalabi AG, Wasef S, Haddad YM, Sadek R, Ijaz K, Peters CJ, Ksiazek TG: An outbreak of Crimean-Congo hemorrhagic fever in the United Arab Emirates, 1994–1995. Am J Trop Med Hyg 1997, 57:519-525.
• [31]Lotfollahzadeh S, Nikbakht Boroujeni GH, Mokhber Dezfouli MR, Bokaei S: A Serosurvey of Crimean-Congo Haemorrhagic Fever Virus in Dairy Cattle in Iran. Zoonoses Public Health 2011, 58:54-59.
• [32]Deyde VM, Khristova ML, Rollin PE, Ksiazek TG, Nichol ST: Crimean-Congo hemorrhagic fever virus genomics and global diversity. J Virol 2006, 80:8834-8842.
• [33]Lindeborg M, Barboutis C, Ehrenborg C, Fransson T, Jaenson TG, Lindgren PE, Lundkvist A, Nyström F, Salaneck E, Waldenström J, Olsen B: Migratory birds, ticks, and crimean-congo hemorrhagic Fever virus. Emerg Infect Dis 2012, 18:2095-2097.
• [34]Abu Salma AA: Meat hygiene in the Sudan: Public health implications of edible offals. MVSc thesis: Faculty of Veterinary Medicine, University of Khartoum, Sudan; 1995.
• [35]Mariner JC, Morill J, Ksiasik TG: Antibodies to hemorrhagic fever viruses in domestic livestock in Niger: Rift Valley fever and Crimean Congo hemorrhagic fever. The American Society of Tropical Medicine and Hygiene. Am J Trop Met Hyg 1995, 53:217-222.