Expression of leukosialin (CD43) defines a major intrahepatic T cell subset associated with protective responses in visceral leishmaniasis

Particle and Fibre Toxicology

Alexandre Morrot¹  Clarisa Beatriz Palatnik-de-Sousa³  Juliany Cola Fernandes Rodrigues²  Adriane Todeschini²  Celio Geraldo Freire-de-Lima²  Andre Macedo Vale²  Joseane Lima Prado Coutinho²  Natália Rodrigues Mantuano²  Erivan Schnaider Ramos-Junior²  Leonardo Santos¹  Naiara Maran¹  Dirlei Nico³ 

[1] Departamento de Imunologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro (UFRJ), CCS - Sala D1-035, Av. Carlos Chagas Filho, 373 - Cidade Universitária, CEP 21.941-902, Ilha do Fundão, Rio de Janeiro, RJ, Brazil;Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Departamento de Microbiologia Geral, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

关键词: Host protective responses;    Intrahepatic T cell subsets;    Leukosialin (CD43);    Leishmania (L.) infantum chagasi;    Visceral leishmaniasis;   

Others  :  1132263
DOI  :  10.1186/s13071-015-0721-9

 received in 2014-11-24, accepted in 2015-02-06,  发布年份 2015

【 摘 要 】

Background

Leishmaniasis is a neglected vector-borne tropical disease caused by Leishmania protozoa that are transmitted to mammalian hosts by infected sand flies. Infection is associated with distinct clinical manifestations that include cutaneous, mucocutaneous and visceral lesions. Visceral leishmaniasis (VL) is the most severe form of the disease and is considered second in terms of mortality and fourth in terms of morbidity among tropical diseases. IFN-γ-producing T cells are involved in protection against the disease.

Methods

CD43^+/+ and CD43^-/- mice on a C57BL/6background were intravenously injected with 5 × 10 ⁷ amastigotes of Leishmania (L.) infantum chagasi, and 30 days after infection the clinical signs of disease were examined; the splenocytes were isolated and assayed for cytokine production; and the livers were removed for phenotypic analysis of T cell subsets by flow cytometry.

Results

We report that mice lacking CD43 display increased susceptibility to infection by Leishmania (L.) infantum chagasi, with higher parasite burdens than wild-type mice. The increased susceptibility of CD43^−/− mice were associated with a weakened delayed hypersensitivity response and reduced levels of IgG2a antibodies to leishmania antigens. We further showed that expression of CD43 defines a major intrahepatic CD4⁺ and CD8⁺ T cell subsets with pro-inflammatory phenotypes and leads to increased levels of IFN-γ secretion by activated splenocytes.

Conclusions

Our findings point to a role of CD43 in the development of host resistance to visceral leishmaniasis.

【 授权许可】

   
2015 Nico et al.; licensee BioMed Central.

【 图 表 】

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