Development of an immunochromatography strip test based on truncated nucleocapsid antigens of three representative hantaviruses

Virology Journal

Jiro Arikawa¹  Clas Ahlm²  Sanae Nishio¹  Kanae Shiokawa¹  Chandika D Gamage¹  Kenta Shimizu¹  Takaaki Koma¹  Kumiko Yoshimatsu¹  Takako Amada¹ 

[1] Department of Microbiology, Graduate School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita ku, Sapporo 060-8683, Japan;Division of Infectious Diseases, Department of Clinical Microbiology, Umeå University, Umeå S-901 85, Sweden

关键词: Hantavirus pulmonary syndrome (HPS);    Nephropathia epidemica (NE);    Hemorrhagic fever with renal syndrome (HFRS);    Immunochromatography strip test;   

Others  :  804498
DOI  :  10.1186/1743-422X-11-87

 received in 2013-11-28, accepted in 2014-04-23,  发布年份 2014

【 摘 要 】

Background

Hantaviruses are causative agents of hemorrhagic fever with renal syndrome (HFRS) and nephropathia epidemica (NE) in the Old World and hantavirus pulmonary syndrome (HPS) in the New World. There is a need for time-saving diagnostic methods. In the present study, recombinant N antigens were used as antigens in an immunochromatography strip (ICG) test to detect specific IgG antibodies.

Methods

The N-terminal 103 amino acids (aa) of Hantaan virus (HTNV), Puumala virus (PUUV) and Andes virus (ANDV) nucleocapsid (N) protein were expressed in E. coli as representative antigens of three groups (HFRS, NE and HPS-causing viruses) of hantavirus. Five different types of ICG test strips, one antigen line on one strip for each of the three selected hantaviruses (HTNV, PUUV and ANDV), three antigen lines on one strip and a mixed antigen line on one strip, were developed and sensitivities were compared.

Results

A total of 87 convalescent-phase patient sera, including sera from 35 HFRS patients, 36 NE patients and 16 HPS patients, and 25 sera from healthy seronegative people as negative controls were used to evaluate the ICG test. Sensitivities of the three-line strip and mixed-line strip were similar to those of the single antigen strip (97.2 to 100%). On the other hand, all of the ICG test strips showed high specificities to healthy donors.

Conclusion

These results indicated that the ICG test with the three representative antigens is an effective serodiagnostic tool for screening and typing of hantavirus infection in humans.

【 授权许可】

   
2014 Amada et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

【 参考文献 】

• [1]Schmaljohn CS, Hooper JW: Bunyaviridae: the viruses and their replication. In Field’s virology. Volume 1. 4th edition. Edited by Fields BN KD, Howley PM. Philadelphia: Lippincott-Raven Publishers; 2002::1581-1601.
• [2]Schmaljohn C, Hjelle B: Hantaviruses: a global disease problem. Emerg Infect Dis 1997, 3:95-104.
• [3]Virus Taxonomy: 2012 release http://www.ictvonline.org/virusTaxonomy.asp webcite
• [4]Watson DC, Sargianou M, Papa A, Chra P, Starakis I, Panos G: Epidemiology of Hantavirus infections in humans: A comprehensive, global overview. Crit Rev Microbiol 2014, 40:261-272.
• [5]Plyusnin A, Vapalahti O, Lundkvist A: Hantaviruses: genome structure, expression and evolution. J Gen Virol 1996, 77:2677-2687.
• [6]Murgue B, Domart Y, Coudrier D, Rollin PE, Darchis JP, Merrien D, Zeller HG: First reported case of imported hantavirus pulmonary syndrome in europe. Emerg Infect Dis 2002, 8:106-107.
• [7]Puljiz I, Kuzman I, Markotic A: Imported case of nephropathia epidemica–a case report. Coll Antropol 2008, 32:1263-1265.
• [8]Reynolds S, Galanis E, Krajden M, Morshed M, Bowering D, Abelson W, Kollmann TR: Imported fatal hantavirus pulmonary syndrome. Emerg Infect Dis 2007, 13:1424-1425.
• [9]Jonsson CB, Figueiredo LT, Vapalahti O: A global perspective on hantavirus ecology, epidemiology, and disease. Clin Microbiol Rev 2010, 23:412-441.
• [10]Rasmuson J, Andersson C, Norrman E, Haney M, Evander M, Ahlm C: Time to revise the paradigm of hantavirus syndromes? Hantavirus pulmonary syndrome caused by European hantavirus. Eur J Clin Microbiol Infect Dis 2011, 30:685-690.
• [11]Hjelle B, Goade D, Torrez-Martinez N, Lang-Williams M, Kim J, Harris RL, Rawlings JA: Hantavirus pulmonary syndrome, renal insufficiency, and myositis associated with infection by Bayou hantavirus. Clin Infect Dis 1996, 23:495-500.
• [12]Koma T, Yoshimatsu K, Pini N, Safronetz D, Taruishi M, Levis S, Endo R, Shimizu K, Yasuda SP, Ebihara H, Feldmann H, Enria D, Arikawa J: Truncated hantavirus nucleocapsid proteins for serotyping Sin Nombre, Andes, and Laguna Negra hantavirus infections in humans and rodents. J Clin Microbiol 2010, 48:1635-1642.
• [13]Araki K, Yoshimatsu K, Ogino M, Ebihara H, Lundkvist A, Kariwa H, Takashima I, Arikawa J: Truncated hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul, and Dobrava hantavirus infections. J Clin Microbiol 2001, 39:2397-2404.
• [14]Kariwa H, Yoshimatsu K, Arikawa J: Hantavirus infection in East Asia. Comp Immunol Microbiol Infect Dis 2007, 30:341-356.
• [15]Vaheri A, Henttonen H, Voutilainen L, Mustonen J, Sironen T, Vapalahti O: Hantavirus infections in Europe and their impact on public health. Rev Med Virol 2013, 23:35-49.
• [16]Amada T, Yoshimatsu K, Yasuda PS, Shimizu K, Koma T, Hayashimoto N, Gamage CD, Nishio S, Takakura A, Arikawa J: Rapid, whole blood diagnostic test for detecting anti-hantavirus antibody in rats. J Virol Methods 2013, 193:42-49.
• [17]Hujakka H, Koistinen V, Eerikainen P, Kuronen I, Laatikainen A, Kauppinen J, Vaheri A, Vapalahti O, Narvanen A: Comparison of a new immunochromatographic rapid test with a commercial EIA for the detection of Puumala virus specific IgM antibodies. J Clin Virol 2001, 23:79-85.
• [18]Hujakka H, Koistinen V, Eerikainen P, Kuronen I, Mononen I, Parviainen M, Lundkvist A, Vaheri A, Narvanen A, Vapalahti O: New immunochromatographic rapid test for diagnosis of acute Puumala virus infection. J Clin Microbiol 2001, 39:2146-2150.
• [19]Lee HW: Hemorrhagic fever with renal syndrome (HFRS). Scand J Infect Dis Suppl 1982, 36:82-85.
• [20]Piiparinen H, Vapalahti O, Plyusnin A, Vaheri A, Lankinen H: Sequence analysis of the Puumala hantavirus Sotkamo strain L segment. Virus Res 1997, 51:1-7.
• [21]Ogino M, Ebihara H, Lee BH, Araki K, Lundkvist A, Kawaoka Y, Yoshimatsu K, Arikawa J: Use of vesicular stomatitis virus pseudotypes bearing hantaan or seoul virus envelope proteins in a rapid and safe neutralization test. Clin Diagn Lab Immunol 2003, 10:154-160.
• [22]Mertens M, Wolfel R, Ullrich K, Yoshimatsu K, Blumhardt J, Romer I, Esser J, Schmidt-Chanasit J, Groschup MH, Dobler G, Essbauer SS, Ulrich RG: Seroepidemiological study in a Puumala virus outbreak area in South-East Germany. Med Microbiol Immunol 2009, 198:83-91.
• [23]Tegshduuren E, Yoshimatsu K, Taruishi M, Endo R, Shimizu K, Koma T, Yasuda SP, Kariwa H, Arikawa J, Ishihara C: Different cross-reactivity of human and rodent sera to Tula virus and Puumala virus. Comp Immunol Microbiol Infect Dis 2010, 33:e67-73.
• [24]Morii M, Yoshimatsu K, Arikawa J, Zhou G, Kariwa H, Takashima I: Antigenic characterization of Hantaan and Seoul virus nucleocapsid proteins expressed by recombinant baculovirus: application of a truncated protein, lacking an antigenic region common to the two viruses, as a serotyping antigen. J Clin Microbiol 1998, 36:2514-2521.
• [25]Elgh F, Lundkvist A, Alexeyev OA, Wadell G, Juto P: A major antigenic domain for the human humoral response to Puumala virus nucleocapsid protein is located at the amino-terminus. J Virol Methods 1996, 59:161-172.
• [26]Yamada T, Hjelle B, Lanzi R, Morris C, Anderson B, Jenison S: Antibody responses to Four Corners hantavirus infections in the deer mouse (Peromyscus maniculatus): identification of an immunodominant region of the viral nucleocapsid protein. J Virol 1995, 69:1939-1943.
• [27]Yoshimatsu K, Arikawa J, Kariwa H: Application of a recombinant baculovirus expressing hantavirus nucleocapsid protein as a diagnostic antigen in IFA test: cross reactivities among 3 serotypes of hantavirus which causes hemorrhagic fever with renal syndrome (HFRS). J Vet Med Sci 1993, 55:1047-1050.
• [28]Miyamoto H, Kariwa H, Araki K, Lokugamage K, Hayasaka D, Cui BZ, Lokugamage N, Ivanov LI, Mizutani T, Iwasa MA, Yoshimatsu K, Arikawa J, Takashima I: Serological analysis of hemorrhagic fever with renal syndrome (HFRS) patients in Far Eastern Russia and identification of the causative hantavirus genotype. Arch Virol 2003, 148:1543-1556.
• [29]Okumura M, Yoshimatsu K, Araki K, Lee BH, Asano A, Agui T, Arikawa J: Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses. Arch Virol 2004, 149:2427-2434.
• [30]Yoshimatsu K, Arikawa J, Tamura M, Yoshida R, Lundkvist A, Niklasson B, Kariwa H, Azuma I: Characterization of the nucleocapsid protein of hantaan virus strain 76–118 using monoclonal antibodies. J gen Virol 1996, 77:695-704.