Multicolor banding remains an important adjunct to array CGH and conventional karyotyping

Molecular Cytogenetics

Caroline Mackie Ogilvie²  Angela F Davies¹  Susan M Bint¹ 

[1] Cytogenetics department, GSTS-Pathology, Guy’s and St. Thomas’ Hospital NHS Foundation Trust, London SE1 9RT, UK;Division of Genetics and Molecular Medicine, King’s College London, School of Medicine at Guy’s, King’s College and St Thomas’ Hospitals, London SE1 9RT, UK

关键词: Complex chromosome rearrangements;    Karyotype analysis;    Array CGH;    Fluorescence in situ hybridization;    Multicolor banding;   

Others  :  1150645
DOI  :  10.1186/1755-8166-6-55

 received in 2013-09-27, accepted in 2013-10-21,  发布年份 2013

【 摘 要 】

Background

Array comparative genomic hybridization (CGH) for high resolution detection of chromosome imbalance, and karyotype analysis using G-banded chromosomes for detection of chromosome rearrangements, provide a powerful diagnostic armoury for clinical cytogenetics. However, abnormalities detected by karyotype analysis cannot always be characterised by scrutinising the G-banded pattern alone, and imbalance detected by array CGH cannot always be visualised in the context of metaphase chromosomes. In some cases further techniques are needed for detailed characterisation of chromosomal abnormalities. We investigated seven cases involving structural chromosome rearrangements detected by karyotype analysis, and one case where imbalance was primarily detected by array CGH. Multicolor banding (MCB) was used in all cases and proved invaluable in understanding the detailed structure of the abnormalities.

Findings

Karyotype analysis detected structural chromosome rearrangements in 7 cases and MCB was used to help refine the karyotype for each case. Array CGH detected imbalance in an eighth case, where previously, G-banded chromosome analysis had reported a normal karyotype. Karyotype analysis of a second tissue type revealed this abnormality in mosaic form; however, MCB was needed in order to characterise this rearrangement. MCB provided information for the delineation of small deletions, duplications, insertions and inversions and helped to assign breakpoints which were difficult to identify from G-banded preparations due to ambiguous banding patterns.

Conclusion

Despite the recent advance of array CGH in molecular cytogenetics we conclude that fluorescence in situ hybridization, including MCB, is still required for the elucidation of structural chromosome rearrangements, and remains an essential adjunct in modern diagnostic laboratories.

【 授权许可】

   
2013 Bint et al.; licensee BioMed Central Ltd.

【 图 表 】

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