Epidemiology of Giardia duodenalis infection in ruminant livestock and children in the Ismailia province of Egypt: insights by genetic characterization

Particle and Fibre Toxicology

Toni Aebischer²  Karl-HZessin⁴  Georg Von Samson-Himmelstjerna⁵  Karsten Nöckler¹  Jürgen Krücken⁵  Hendrik Wilking³  Christian Klotz²  Yosra AHelmy⁴ 

[1] Federal Institute for Risk Assessment (BfR), 12277 Berlin, Germany;Mycotic and parasitic agents and mycobacteria, Department of Infectious Diseases, Robert Koch-Institute, 13353 Berlin, Germany;Gastrointestinal Infections, Zoonoses and Tropical Infections, Department of Infectious Disease Epidemiology, Robert Koch-Institute, 13353 Berlin, Germany;Faculty Panel Veterinary Public Health, Freie Universität Berlin, 14163 Berlin, Germany;Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany

关键词: Egypt;    Spatial distribution;    β-giardin (bg);    Glutamate dehydrogenase (gdh);    Triose phosphate isomerase (tpi);    RIDA^®QUICK test;    Real time PCR;    Diarrheal children;    Ruminants;    Genetic characterization;    Giardia;   

Others  :  1183558
DOI  :  10.1186/1756-3305-7-321

 received in 2014-01-15, accepted in 2014-07-05,  发布年份 2014

【 摘 要 】

Background

Giardia duodenalis is a common flagellated protozoan parasite that infects the small intestine of a wide range of vertebrate hosts. This study aimed to determine whether tracing of G. duodenalis isolates by current genetic typing tools is possible using an exemplary set of samples from infected cattle, buffalo and children from the Ismailia province, Egypt.

Method

A total of 804 fecal samples from ruminant animals was collected from 191 herds and 165 samples from diarrheal children below the age of 10 years. Parasites were detected in these samples using the copro-antigen RIDA^®QUICK test and by real-time PCR. Samples were then genetically characterized based on the triosephosphate isomerase, glutamate dehydrogenase and β-giardin genes.

Results

The prevalence of G. duodenalis was 53% in ruminants and 21% in symptomatic children and infection was not positively correlated with diarrheal symptoms. Sequence typing analysis confirmed predominance of B-type sequences (>67%) in humans and E-type sequences (>81%) in ruminants over A-type sequences. For 39 samples the complete sequence information of the three marker gene fragments could be derived. Integration of the concatenated sequence information of the three marker gene fragments with the spatial data of the respective sample revealed that identical or near identical (only up to 1 out of 1358 bp different) concatenated sequencing types were spatially related in 4 out of 5 cases.

Conclusion

The risk of zoonotic infection emanating from ruminants even in high prevalence areas is negligible. Genetic characterization indicated a predominant anthropogenic cycle of infection within the pediatric population studied. Integration of sequence typing data with information on geographic origins of samples allows parasite sub-population tracing using current typing tools.

【 授权许可】

   
2014 Helmy et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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