Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc-145 cell

Virology Journal

Guang-Zhi Tong¹  Yi-Feng Jiang¹  Ling Li¹  Wu Tong¹  Shan-Rui Zhang¹  Yan-Jun Zhou¹  Yan-Zhao Xu¹ 

[1] Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518, Ziyue Road, Minhang District, Shanghai, 200241, China

关键词: Deletion mutant;    Nonessential region;    Nsp2;    HuN4-F112;   

Others  :  1154291
DOI  :  10.1186/1743-422X-9-141

 received in 2011-11-07, accepted in 2012-07-25,  发布年份 2012

【 摘 要 】

Background

The regions in the middle of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus (PRRSV) have been shown to be nonessential for PRRSV replication, and these nonessential regions are different in various viral strains.

Finding

In this study, the nonessential regions of the nsp2 of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and respiratory syndrome virus were identified based on an infectious cDNA clone of HuN4-F112. The results demonstrated that the segments of nsp2 [amino acids (aa) 480 to 667] tolerated deletions. Characterization of the mutants demonstrated that those with small deletions did not affect the viral growth on Marc-145 cells, but deletion of these regions led to earlier PRRSV replication increased (before 36 h after infectious in vitro).

Conclusion

The functional roles of nsp2 variable middle region for PRRSV HuN4-F112 replication have been identified. Our results also suggested that none-essential region might be an ideal insertion region to express foreign gene in PRRSV genome.

【 授权许可】

   
2012 Xu et al.; licensee BioMed Central Ltd.

【 图 表 】

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