Distribution of Rickettsia rickettsii in ovary cells of Rhipicephalus sanguineus (Latreille1806) (Acari: Ixodidae)

Particle and Fibre Toxicology

Maria Izabel Camargo-Mathias¹  Marcelo Bahia Labruna²  João Fábio Soares²  Pablo Henrique Nunes¹  Luís Flávio da Silva Costa¹ 

[1] Departamento de Biologia, Instituto de Biociências UNESP - Rio Claro/SP, Brasil;Laboratório de Doenças Parasitárias do Departamento de Medicina Veterinária Preventiva e Saúde Animal - VPS, USP - São Paulo, Brasil

关键词: histology;    ovary;    Brazilian Spotted Fever;    Rhipicephalus sanguineus;    Rickettsia rickettsii;   

Others  :  1235312
DOI  :  10.1186/1756-3305-4-222

 received in 2011-09-15, accepted in 2011-11-25,  发布年份 2011

【 摘 要 】

Background

Considering the fact that the dog tick, Rhipicephalus sanguineus, has a great potential to become the vector of Brazilian Spotted Fever (BSF) for humans, the present study aimed to describe the distribution of the bacterium Rickettsia rickettsii, the etiological agent of BSF, in different regions of the ovaries of R. sanguineus using histological techniques. The ovaries were obtained from positive females confirmed by the hemolymph test and fed in the nymph stage on guinea pigs inoculated with R. rickettsii.

Results

The results showed a general distribution of R. rickettsii in the ovary cells, being found in oocytes in all stages of development (I, II, III, IV and V) most commonly in the periphery of the oocyte and also in the cytoplasm of pedicel cells.

Conclusions

The histological analysis of the ovaries of R. sanguineus infected females confirmed the presence of the bacterium, indicating that the infection can interfere negatively in the process of reproduction of the ticks, once alterations were detected both in the shape and cell structure of the oocytes which contained bacteria.

【 授权许可】

   
2011 da Silva Costa et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

【 参考文献 】

• [1]Melles HHB, Colombo S, Lemos ERS: Isolamento de Rickettsia em cultura de células Vero. Rev Soc Bras Med Trop 1999, 32(Suppl 5):469-473.
• [2]Vranjac A: Informe Técnico Sobre a Febre Maculosa Brasileira. Secretaria de Estado da Saúde Coordenação dos Institutos de Pesquisa Divisão de Zoonoses, São Paulo 2002.
• [3]La Scola B, Raoult D: Laboratory diagnosis of rickettsioses: current approaches to diagnosis of old and new rickettsial diseases. J clin microbiol 1997, 35(Suppl 11):2715-2727.
• [4]Mcdade JE: Rickettsial diseases. In Microbiology and Microbial Infections. 9th edition. Edited by Hausler Jr WJ, Sussman M. London: Topley and Wilson's; 1998:995-1011.
• [5]Silva ME: Frequência De Anticorpos Anti-Rickettsia Spp Em Cães Da Cidade De Belo Horizonte. Dissertação de mestrado apresentada à Universidade Federal de Minas Gerais UFMG, Faculdade de Veterinária de Belo Horizonte 2007.
• [6]Cardoso LD: Detecção e caracterização da Rickettsia spp circulante em foco inativo periurbano do município de Caratinga, MG. Dissertação de Mestrado apresentada à Universidade Federal de Ouro Preto, Núcleo de Pesquisa em Ciências Biológicas Programa de Pós-graduação em Ciências Biológicas 2004.
• [7]Labruna MB: Ecology of Rickettsia in South America. In Rickettsiology and Rickettsial Diseases-Fifth International Conference. Volume 1166. Edited by Hechmy KE, Samuel JE, Brouqui P, Raoult DA. Annals of the New York Academy of Sciences: May 2009; New York; 2009::156-166.
• [8]Labruna MB, Ogrzewalska M, Martins TF, Pinter A, Horta MC: Comparative Susceptibility of Larval Stages of Amblyomma aureolatum, Amblyomma cajennense, and Rhipicephalus sanguineus to Infection by Rickettsia rickettsii. J med entomol 2008, 45(Suppl 6):1156-1159.
• [9]Pinter A, Labruna MB: Isolation of Rickettsia rickettsii and Rickettsia bellii in cell culture from the tick Amblyomma aureolatum in Brazil. Ann NY Acad Sci 2006, 1078:523-529.
• [10]Moraes-Filho J, Pinter A, Pacheco RC, Gutmann TB, Barbosa SO, Gonzáles MARM, Cecílio SRM, Labruna MB: New Epidemiological Data on Brazilian Spotted Fever in an Endemic Area of the State of São Paulo, Brazil. Vector Borne Zoonotic Dis 2009, 9(Suppl 1):73-78.
• [11]Cunha NC, Fonseca AH, Rezende J, Rozental T, Favacho ARM, Barreira JD, Massard CL, Lemos ERS: First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro. Pesqui Vet Bras 2009, 29(Suppl 2):105-108.
• [12]Pacheco RC, Moraes-Filho J, Guedes E, Silveira I, Richtzenhain LJ, Leite RC, Labruna MB: Rickettsial Infections of Dogs, Horses and Ticks In Juiz de Fora, Southeastern Brazil, and Isolation of Rickettsia rickettsii from Rhipicephalus sanguineus Ticks. Med vet entomol 2011, 25:148-155.
• [13]Demma LJ, Traeger MS, Nicholson WL, Paddock DR, Blau DM, Eremeeva ME, Dasch GA, Levin MJ, Singleton J Jr, Zaki SR, Cheek JE, Swerdlow DL, Mcquiston JH: Rocky Mountain Spotted Fever from an Unexpected Tick Vector in Arizona. N Engl J Med 2005, 353(Suppl 6):587-594.
• [14]Dantas-Torres F, Figueredo LA, Brandão-Filho SP: Rhipicephalus sanguineus (Acari: Ixodidae), The Brown Dog Tick, Parasitizing Humans in Brazil. Rev So Bras Med Trop 2006, 39(Suppl 1):64-67.
• [15]Louly CCB, Fonseca IN, Oliveira VF, Borges LMF: Ocorrência de Rhipicephalus sanguineus em Trabalhadores de Clínicas Veterinárias e Canis, no Município de Goiânia, GO. Ci Anim Bras 2006, 7(Suppl 1):103-106.
• [16]Pegram RG, Clifford CM, Walker JB, Keirans JE: Classification of the Rhipicephalus sanguineus, Rhipicephalus sulcatus and Rhipicephalus turanicus. Syst Parasitol 1987, 10:3-26.
• [17]Sexton DJ, Burgdorfer W, Thomas L: Rocky Mountain spotted fever in Mississipi: survey for spotted fever antibodies in dogs and for spotted fever group Rickettsial in dog ticks. Am J Epidemiol 1976, 103:192-197.
• [18]Labruna MB, Pereira MC: Carrapatos em cães no Brasil. Clin Vet 2001, 30:24-32.
• [19]Oliveira PR, Bechara GH, Denardi SE, Saito KC, Nunes ET, Camargo-Mathias MI: Morphological characterization of the ovary and oocytes vitellogenesis of the tick Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae). Exp Parasitol 2005, 110:146-156.
• [20]Burgdorfer W: The hemolymph test. Am J Trop Med Hyg 1970, 19:1010-1014.
• [21]Mancini DAP, Nascimento EMM, Tavares VR, Soares MA: A ocorrência de riquetsioses do gruporickettsia rickettsii. Rev Saúde Públ 1983, 17:493-499.
• [22]Parker R, Philip CB, Jellinson WL: Rocky Mountain spotted fever. Potentialities of tick transmission in relation to geographic occurrence in the United States. Am J Trop Med 1933, 13:241-378.
• [23]Piranda EM, Faccini JLH, Pinter A, Pacheco RC, Cançado PHD, Labruna MB: Experimental Infection of Rhipicephalus sanguineus Ticks with the Bacterium Rickettsia rickettsii, Using Experimentally Infected Dogs. Vector Borne Zoonotic Dis 2011, 11(Suppl 1):29-35.
• [24]Burgdorfer W, Brinton LP: Mechanisms of transovarial infec-tion of spotted fever rickettsiae in ticks. Ann N Y Acad Sci 1975, 266:61-72.
• [25]Burgdorfer W: Investigation of "Transovarial Transmission" of Rickettsia rickettsii in the Wood Tick, Dermacentor andersoni. Exp Parasitol 1963, 14:152-159.
• [26]Mcdade JE, Newhouse VF: Natural History of Rickettsia rickettsii. Ann Rev Microbiol 1986, 40:287-309.
• [27]Labruna MB, Whitworth T, Horta MC, Bouyer DH, Mcbride JW, Pinter A, Popov V, Gennari SM, Walker DH: Rickettsia species infecting Amblyomma cooperi ticks from an area in state of São Paulo, Brazil, where Brazilian spotted fever is endemic. J clin microbiol 2004, 42:90-98.
• [28]Giménez DF: Staining rickettsiae in yolk sacs cultures. Stain Technol 1964, 39:135-140.