Larvicidal efficacy of Cryptomeria japonica leaf essential oils against Anopheles gambiae

Particle and Fibre Toxicology

Eliningaya J Kweka⁴  Shang-Tzen Chang¹  Gamba Nkwengulila³  Lucile Lyaruu⁵  Sen-Sung Cheng²  France P Mdoe³ 

[1] School of Forestry and Resource Conservation, National Taiwan University, No. 1, Sec.4, Roosevelt Road, Taipei 106, Taiwan;Experimental Forest, National Taiwan University, Nan-Tou 557, Taiwan;Department of Zoology and Wildlife Conservation, College of Natural and Applied Sciences, University of Dar-es-salaam, P.O. Box 35165, Dar-es-salaam, Tanzania;Department of Medical Parasitology and Entomology, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza, Tanzania;Division of Livestock and Human Diseases Vector Control, Tropical Pesticides Research Institute, Ngaramtoni, Off Nairobi Road, P.O. Box 3024, Arusha, Tanzania

关键词: Essential oil;    Mortality;    Anopheles gambiae s.s;    Larvicidal efficacy;    Cryptomeria japonica;   

Others  :  1150398
DOI  :  10.1186/1756-3305-7-426

 received in 2014-05-02, accepted in 2014-09-01,  发布年份 2014

【 摘 要 】

Background

Alternative insecticidal compounds with mortality effect against mosquito life cycle stages are currently needed. The compounds should be biodegradable and nontoxic to non-targeted insects. Plant based larvicides provide effective control of vector populations. This study explored Cryptomeria japonica leaf essential oil larvicidal potency against Anopheles gambiae sensu stricto.

Methods

Essential oils (12.5 to 200 μg/mL) extracted from C. japonica leaves were evaluated against An. gambiae s.s. larvae in both the laboratory and semi field in 6 replicates for each dose. Larval mortality readings were taken at 12, 24, 48, and 72 h post treatment.

Results

C. japonica leaf essential oil yield was 17.06 ± 0.56 mL/kg and 1.60 ± 0.33% (w/w). GC-FID and GC-MS analyses revealed 22 constituents. Essential oil was more effective against An. gambiae s.s. larvae in the laboratory than in semi field trials. Mortality increased with increasing dosages (12.5 to 200 μg/mL) in the laboratory (31.75 to 100%) and semi field trials (17.75 to 99.5%), respectively. The LC₅₀ value ranged from 5.55 to 63.92 μg/mL in the laboratory, and 8.22 to 134.84 μg/mL in semi field conditions, LC₉₀ value ranged from 41.34 to 205.93 μg/mL in the laboratory and 50.92 to 213.11 μg/mL in semi field conditions.

Conclusion

This study has demonstrated the potential of C. japonica leaf essential oil to cause mortality effects to An. gambiae s. s. larval populations, however, further studies need to be conducted under field conditions and also with individual active compounds of C. japonica essential oil.

【 授权许可】

   
2014 Mdoe et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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