Novel genes upregulated when NOTCH signalling is disrupted during hypothalamic development

Neural Development

Valérie Dupé¹  Véronique David²  Christèle Dubourg²  Isabelle Gicquel¹  Hélène Romé¹  Frédérique Barloy-Hubler³  Michelle Ware¹  Leslie Ratié¹ 

[1] Institut de Génétique et Développement de Rennes, CNRS UMR6290, Université de Rennes 1, IFR140 GFAS, Faculté de Médecine, Rennes, France;Laboratoire de Génétique Moléculaire, CHU Pontchaillou, Rennes, France;Amadeus Platform, SFR BIOSIT, Rennes, France

关键词: Embryonic brain;    Neuronal differentiation;    Hypothalamus;    Proneural genes;    Notch;   

Others  :  803544
DOI  :  10.1186/1749-8104-8-25

 received in 2013-10-18, accepted in 2013-12-10,  发布年份 2013

【 摘 要 】

Background

The generation of diverse neuronal types and subtypes from multipotent progenitors during development is crucial for assembling functional neural circuits in the adult central nervous system. It is well known that the Notch signalling pathway through the inhibition of proneural genes is a key regulator of neurogenesis in the vertebrate central nervous system. However, the role of Notch during hypothalamus formation along with its downstream effectors remains poorly defined.

Results

Here, we have transiently blocked Notch activity in chick embryos and used global gene expression analysis to provide evidence that Notch signalling modulates the generation of neurons in the early developing hypothalamus by lateral inhibition. Most importantly, we have taken advantage of this model to identify novel targets of Notch signalling, such as Tagln3 and Chga, which were expressed in hypothalamic neuronal nuclei.

Conclusions

These data give essential advances into the early generation of neurons in the hypothalamus. We demonstrate that inhibition of Notch signalling during early development of the hypothalamus enhances expression of several new markers. These genes must be considered as important new targets of the Notch/proneural network.

【 授权许可】

   
2013 Ratié et al.; licensee BioMed Central Ltd.

【 图 表 】

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