Isolation and characterization of a novel alphanodavirus

Virology Journal

Xinwen Chen³  Zhengli Shi³  Shili Han⁴  Yan Li⁵  Haitao Mao¹  Xiang Li²  Yun Wang³  Huimin Bai⁵ 

[1] Institut Pasteur of Shanghai, Chinese Academy of Sciences, Viral Hepatitis Research Unit, Shanghai, 200025, China;Department of Cell Biology, Wenzhou Medical College, Wenzhou, 325035, China;State Key Lab of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China;College of Life Sciences, Huazhong Normal University, Wuhan, 430079, China;Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China

关键词: Host susceptibility;    Hz-AM1;    HzNV;    Alphanodavirus;   

Others  :  1156526
DOI  :  10.1186/1743-422X-8-311

 received in 2011-03-01, accepted in 2011-06-19,  发布年份 2011

【 摘 要 】

Background

Nodaviridae is a family of non-enveloped isometric viruses with bipartite positive-sense RNA genomes. The Nodaviridae family consists of two genera: alpha- and beta-nodavirus. Alphanodaviruses usually infect insect cells. Some commercially available insect cell lines have been latently infected by Alphanodaviruses.

Results

A non-enveloped small virus of approximately 30 nm in diameter was discovered co-existing with a recombinant Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) in Hz-AM1 cells. Genome sequencing and phylogenetic assays indicate that this novel virus belongs to the genus of alphanodavirus in the family Nodaviridae and was designated HzNV. HzNV possesses a RNA genome that contains two segments. RNA1 is 3038 nt long and encodes a 110 kDa viral protein termed protein A. The 1404 nt long RNA2 encodes a 44 kDa protein, which exhibits a high homology with coat protein precursors of other alphanodaviruses. HzNV virions were located in the cytoplasm, in association with cytoplasmic membrane structures. The host susceptibility test demonstrated that HzNV was able to infect various cell lines ranging from insect cells to mammalian cells. However, only Hz-AM1 appeared to be fully permissive for HzNV, as the mature viral coat protein essential for HzNV particle formation was limited to Hz-AM1 cells.

Conclusion

A novel alphanodavirus, which is 30 nm in diameter and with a limited host range, was discovered in Hz-AM1 cells.

【 授权许可】

   
2011 Bai et al; licensee BioMed Central Ltd.

【 图 表 】

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