Genomic alterations in rectal tumors and response to neoadjuvant chemoradiotherapy: an exploratory study

Radiation Oncology

Daniele Calistri¹  Dino Amadori³  Massimo Guidoboni⁶  Luca Saragoni⁷  Paola Rosetti³  Alessandro Passardi³  Enrico Lucci⁸  Ermanno Emiliani⁵  Wainer Zoli¹  Massimo Giannini⁴  Nazario Teodorani⁵  Michela Ballardini²  Chiara Molinari¹ 

[1] Biosciences Laboratories, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (I.R.S.T.), Meldola, Italy;Unit of Biostatistics and Clinical Trials, I.R.S.T., Meldola, Italy;Department of Medical Oncology, I.R.S.T., Meldola, Italy;Radiotherapy Unit, I.R.S.T., Meldola, Italy;Radiotherapy Unit, Santa Maria delle Croci Hospital, Ravenna, Italy;Immunotherapy and Cell Therapy Laboratory, I.R.S.T., Meldola, Italy;Pathology Unit, Morgagni-Pierantoni Hospital, Forlì, Italy;Department of General Surgery, Morgagni-Pierantoni Hospital, Forlì, Italy

关键词: ArrayCGH;    neoadjuvant chemoradiotherapy;    rectal cancer;    Genomic alterations;   

Others  :  1160937
DOI  :  10.1186/1748-717X-6-161

 received in 2011-07-28, accepted in 2011-11-18,  发布年份 2011

【 摘 要 】

Background

Neoadjuvant chemoradiotherapy is the treatment of choice in advanced rectal cancer, even though there are many patients who will not benefit from it. There are still no effective methods for predicting which patients will respond or not. The present study aimed to define the genomic profile of rectal tumors and to identify alterations that are predictive of response in order to optimize therapeutic strategies.

Methods

Forty-eight candidates for neoadjuvant chemoradiotherapy were recruited and their pretherapy biopsies analyzed by array Comparative Genomic Hybridization (aCGH). Pathologic response was evaluated by tumor regression grade.

Results

Both Hidden Markov Model and Smoothing approaches identified similar alterations, with a prevalence of DNA gains. Non responsive patients had a different alteration profile from responsive ones, with a higher number of genome changes mainly located on 2q21, 3q29, 7p22-21, 7q21, 7q36, 8q23-24, 10p14-13, 13q12, 13q31-34, 16p13, 17p13-12 and 18q23 chromosomal regions.

Conclusions

This exploratory study suggests that an in depth characterization of chromosomal alterations by aCGH would provide useful predictive information on response to neoadjuvant chemoradiotherapy and could help to optimize therapy in rectal cancer patients.

The data discussed in this study are available on the NCBI Gene Expression Omnibus [GEO: GSE25885].

【 授权许可】

   
2011 Molinari et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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