Low risk for transmission of zoonotic Giardia duodenalis from dogs to humans in rural Cambodia

Particle and Fibre Toxicology

Rebecca J Traub⁴  Sinuon Muth¹  Virak Khieu¹  Wissanuwat Chimnoi²  Anders Dalsgaard³  Peter Odermatt⁵  Fabian Schär⁵  Tawin Inpankaew² 

[1] National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia;Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand;Department of Veterinary Disease Biology, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark;Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia;University of Basel, Basel, Switzerland

关键词: Cambodia;    Zoonosis;    Dogs;    Humans;    Giardia duodenalis;   

Others  :  1150542
DOI  :  10.1186/1756-3305-7-412

 received in 2014-05-13, accepted in 2014-08-19,  发布年份 2014

【 摘 要 】

Background

A number of epidemiological studies have demonstrated Giardia as prevalent in both humans and dogs worldwide and have postulated the occurrence of anthroponotic, zoonotic and animal-specific cycles of transmission, which may be geographically and regionally unique in its epidemiology. The aim of this study was to utilise molecular tools to determine the prevalence and compare genotypes of Giardia duodenalis infecting humans and dogs living in a previously identified Giardia-endemic village in rural Cambodia in order to ascertain zoonotic transmission risk.

Findings

The prevalence of G. duodenalis in humans and dogs was 18.3% (40/218) and 10.6% (10/94) by PCR, respectively. Molecular characterisation of the small subunit of ribosomal RNA (SSU rRNA) gene, triose phosphate isomerase (TPI) gene and sub-assemblage characterisation of the glutamate dehydrogenase (gdh) gene placed 27.5% (11/40) of Giardia positive humans into assemblage AII and 72.5% (29/40) into assemblage BIII of G. duodenalis. In dogs, 20.0% (2/10) of Giardia-positive samples were characterised as G. duodenalis assemblage BIII, 40.0% (4/10) as assemblage C and 40.0% (4/10) as mix infection between assemblage C and D.

Conclusion

Overall, just over 2% of dogs harboured potentially zoonotic assemblages of G. duodenalis in the studied communities and hence pose a minimal zoonotic risk for the transmission of Giardia to humans.

【 授权许可】

   
2014 Inpankaew et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]van der Giessen JW, de Vries A, Roos M, Wielinga P, Kortbeek LM, Mank TG: Genotyping of Giardia in Dutch patients and animals: a phylogenetic analysis of human and animal isolates. Int J Parasitol 2006, 36:849-858.
• [2]Ryan U, Cacciò SM: Zoonotic potential of Giardia. Int J Parasitol 2013, 43:943-956.
• [3]Inpankaew T, Traub R, Thompson RC, Sukthana Y: Canine parasitic zoonoses in Bangkok temples. Southeast Asian J Trop Med Public Health 2007, 38:247-255.
• [4]Traub RJ, Monis PT, Robertson I, Irwin P, Mencke N, Thompson RC: Epidemiological and molecular evidence supports the zoonotic transmission of Giardia among humans and dogs living in the same community. Parasitology 2004, 128:253-262.
• [5]Lee KJ, Bae YT, Kim DH, Deung YK, Ryang YS, Kim HJ, Im KI, Yong TS: Status of intestinal parasites infection among primary school children in Kampongcham, Cambodia. Korean J Parasitol 2002, 40:153-155.
• [6]Park SK, Kim DH, Deung YK, Kim HJ, Yang EJ, Lim SJ, Ryang YS, Jin D, Lee KJ: Status of intestinal parasite infections among children in Bat Dambang, Cambodia. Korean J Parasitol 2004, 42:201-203.
• [7]Chhakda T, Muth S, Socheat D, Odermatt P: Intestinal parasites in school-aged children in villages bordering Tonle Sap Lake, Cambodia. Southeast Asian J Trop Med Public Health 2006, 37:859-864.
• [8]Moore CE, Hor PC, Soeng S, Sun S, Lee SJ, Parry CM, Day NP, Stoesser N: Changing patterns of gastrointestinal parasite infections in Cambodian children: 2006-2011. J Trop Pediatr 2012, 58:509-512.
• [9]Beck R, Sprong H, Pozio E, Cacciò SM: Genotyping Giardia duodenalis isolates from dogs: lessons from a multilocus sequence typing study. Vector Borne Zoonotic Dis 2012, 12:206-213.
• [10]Schär F, Inpankaew T, Traub RJ, Khieu V, Dalsgaard A, Chimnoi W, Chamnan C, Sok D, Marti H, Muth S, Odermatt P: The prevalence and diversity of intestinal parasitic infections in humans and domestic animals in a rural Cambodian village. Parasitol Int 2014, 63:597-603.
• [11]Hopkins RM, Meloni BP, Groth DM, Wetherall JD, Reynoldson JA, Thompson RC: Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality. J Parasitol 1997, 83:44-51.
• [12]Read C, Walters J, Robertson ID, Thompson RC: Correlation between genotype of Giardia duodenalis and diarrhoea. Int J Parasitol 2002, 32:229-231.
• [13]Sulaiman IM, Fayer R, Bern C, Gilman RH, Trout JM, Schantz PM, Das P, Lal AA, Xiao L: Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis. Emerg Infect Dis 2003, 9:1444-1452.
• [14]Lebbad M, Mattsson JG, Christensson B, Ljungström B, Backhans A, Andersson JO, Svärd SG: From mouse to moose: multilocus genotyping of Giardia isolates from various animal species. Vet Parasitol 2010, 168:231-239.
• [15]Cacciò SM, Beck R, Lalle M, Marinculic A, Pozio E: Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B. Int J Parasitol 2008, 38:1523-1531.
• [16]Ballweber LR, Xiao L, Bowman DD, Kahn G, Cama VA: Giardiasis in dogs and cats: update on epidemiology and public health significance. Trends Parasitol 2010, 26:180-189.
• [17]Traub RJ, Inpankaew T, Reid SA, Sutthikornchai C, Sukthana Y, Robertson ID, Thompson RC: Transmission cycles of Giardia duodenalis in dogs and humans in Temple communities in Bangkok–a critical evaluation of its prevalence using three diagnostic tests in the field in the absence of a gold standard. Acta Trop 2009, 111:125-132.
• [18]Dado D, Montoya A, Blanco MA, Miró G, Saugar JM, Bailo B, Fuentes I: Prevalence and genotypes of Giardia duodenalis from dogs in Spain: possible zoonotic transmission and public health importance. Parasitol Res 2012, 111:2419-2422.
• [19]Liu A, Zhang X, Zhang L, Wang R, Li X, Shu J, Zhang X, Shen Y, Zhang W, Ling H: Occurrence of bovine giardiasis and endemic genetic characterization of Giardia duodenalis isolates in Heilongjiang Province, in the Northeast of China. Parasitol Res 2012, 111:655-661.
• [20]Schurer JM, Hill JE, Fernando C, Jenkins EJ: Sentinel surveillance for zoonotic parasites in companion animals in indigenous communities of Saskatchewan. Am J Trop Med Hyg 2012, 87:495-498.
• [21]Mark-Carew MP, Adesiyun AA, Basu A, Georges KA, Pierre T, Tilitz S, Wade SE, Mohammed HO: Characterization of Giardia duodenalis infections in dogs in Trinidad and Tobago. Vet Parasitol 2013, 196:199-202.
• [22]Solarczyk P, Majewska AC: A survey of the prevalence and genotypes of Giardia duodenalis infecting household and sheltered dogs. Parasitol Res 2010, 106:1015-1019.
• [23]Inpankaew T, Schär F, Dalsgaard A, Khieu V, Chimnoi W, Chhoun C, Sok D, Marti H, Muth S, Odermatt P, Traub RJ: High prevalence of Ancylostoma ceylanicum hookworm infections in humans, Cambodia, 2012. Emerg Infect Dis 2014. http://dx.doi.org/10.3201/eid2006.131770 webcite
• [24]Mohamed AS, Glickman LT, Camp JW Jr, Lund E, Moore GE: Prevalence and risk factors for Giardia spp. infection in a large national sample of pet dogs visiting veterinary hospitals in the United States (2003-2009). Vet Parasitol 2013, 195:35-41.
• [25]Sulaiman IM, Jiang J, Singh A, Xiao L: Distribution of Giardia duodenalis genotypes and subgenotypes in raw urban wastewater in Milwaukee, Wisconsin. Appl Environ Microbiol 2004, 70:3776-3780.
• [26]Cooper MA, Sterling CR, Gilman RH, Cama V, Ortega Y, Adam RD: Molecular analysis of household transmission of Giardia lamblia in a region of high endemicity in Peru. J Infect Dis 2010, 202:1713-1721.