Molecular detection of Torque teno virus in different breeds of swine

Virology Journal

Yingshun Zhou¹  Zhongbing Guan¹  Xin Yang¹  Hongning Wang¹  Zhiwei Wu¹ 

[1] School of Life science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,"985"Project Science Innovative Platform for Resource and environment Protection of Southwestern, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Chengdu, Sichuan, 610065, P.R. China

关键词: Torque teno virus (TTV);    swine breeds;    serums;    nested polymerase chain reaction (nested PCR);   

Others  :  1155683
DOI  :  10.1186/1743-422X-8-503

 received in 2011-08-29, accepted in 2011-11-03,  发布年份 2011

【 摘 要 】

Background

Torque teno virus (TTV), of the Anelloviridae family, Iotatorquevirus genus, is a non-enveloped, single-stranded, and negative sense DNA (ssDNA) virus infecting human and many domestic animals including swines. Very little information is known about the investigations of TTV prevalence in different swine breeds so far.

Methods

In this study, 208 serum samples collected from seven swine breeds (Rongchang pig, Chenghua pig, Zibet pig, Wild boar, Duroc, Landrace, Large Yorkshire) from two independent farms were detected to determine the prevalence of two swine TTV genogroups, TTV1 and TTV 2, by nested polymerase chain reaction methods, and to analyse prevalence difference among these breeds.

Results

The results showed that the prevalence of TTV in the seven breeds was 92%-100%. No significant difference (p > 0.05) in TTV infection was observed between different breeds. Interestingly, significantly higher prevalence for TTV1 in Rongchang boars (90%) and for TTV2 in Rongchang sows (95%) were detected, while co-infection rate (43.8%) was lower than other breeds. Sequence analysis showed that the homology of TTV1 and TTV2 were over 90.9% and 86.4% in these breeds, respectively.

Conclusions

The results indicated that TTV was widely distributed in the seven swine breeds. The prevalence of both TTV genogroups associated with swine breeds and genders. This study also respented the first description of swine TTV prevalence in different swine breeds. It was vitally necessary to further study swine TTV pathogenicity.

【 授权许可】

   
2011 Wu et al; licensee BioMed Central Ltd.

【 图 表 】

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