The IgE response to Ascaris molecular components is associated with clinical indicators of asthma severity

World Allergy Organization Journal

Luis Caraballo²  Alvaro Alvarez¹  Dilia Mercado³  Josefina Zakzuk²  Emiro Buendía³ 

[1] Department of Microbiology, Faculty of Medicine, University of Cartagena, Cartagena, Colombia;Foundation for the Development of Medical and Biological Sciences (Fundemeb), Cartagena, Colombia;Institute for Immunological Research, University of Cartagena, Cartagena, Colombia

关键词: Urban;    Poverty;    Ascariasis;    Dermatophagoides pteronyssinus;    Blomia tropicalis;    Atopy;    Lung function;    The tropics;    IgE;    House dust mites;    Ascaris;    Asthma severity;   

Others  :  1137512
DOI  :  10.1186/s40413-015-0058-z

 received in 2014-10-02, accepted in 2015-01-21,  发布年份 2015

【 摘 要 】

Background

Asthma is a common chronic disease worldwide and Ascaris lumbricoides infection (ascariasis) is frequent in tropical regions. However, the effect of ascariasis on asthma severity has not been sufficiently explored. We sought to evaluate the influence of the IgE immune response to Ascaris extract and purified house dust mites (HDM) and Ascaris allergens on indicators of asthma severity in patients living in the tropics.

Methods

Asthmatic patients from Cartagena, Colombia were recruited. Clinical assessment included questionnaires, physical examination, allergy skin tests, spirometry, parasite stool examination and IgE antibody measurements. Asthma was diagnosed by a physician according to validated criteria. Indicators of severity were occurrence of severe dyspnea episodes, night awakenings events, > 4 emergency room (ER) visits and hospitalizations during the last year. Specific IgE to Der p 2, Ascaris spp., Blomia tropicalis and Dermatophagoides pteronyssinus extracts was determined by ImmunoCap. IgE to tropomyosins (Asc l 3, Blo t 10 and Der p 10), Blo t 5 and Asc s 1 was detected by ELISA. Logistic regression analyses were used to explore the relationships between sensitization and indicators of asthma severity.

Results

After adjustment for HDM sensitization, Ascaris sensitization remained associated with severe dyspnea (aOR: 1.90, 95% CI: 1.08 - 3.34, p = 0.03) and > 4 ER visits (aOR: 2.23, 95% CI: 1.15 - 4.30, p = 0.02). We also found that sensitization to the species specific markers Blo t 5 and Asc s 1, as well as the cross-reactive tropomyosins of D. pteronyssinus and Ascaris were associated with > 4 ER visits. Der p 2 sensitization was associated with bronchodilator responsiveness (aOR: 2.24: 1.25-4.02, p = 0.01). Remarkably, significantly higher IgE levels to HDM species specific allergens were found in Ascaris sensitized patients.

Conclusions

In this tropical population, IgE sensitization to Ascaris and the cross-reactive tropomyosins was frequent and associated with clinical indicators of asthma severity. The significant relationship between sensitization to the nematode-specific marker Asc s 1 and ER attendance supports these findings. Moreover, ascariasis increases the human IgE responses to HDM specific allergens.

【 授权许可】

   
2015 Buendía et al.; licensee BioMed Central.

【 图 表 】

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