Comprehensive analysis of clinical significance of stem-cell related factors in renal cell cancer

World Journal of Surgical Oncology

Shujie Xia¹  Libin Zhou¹  Bingde Yin¹  Lei Xiao²  Jie Fan¹  Changcun Zhang¹  Yongchao Liu¹ 

[1] Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China;Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Cell Bank, Stem Cell Bank, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

关键词: Kidney Neoplasm;    NANOG;    OCT4;    SOX2;    KLF4;    LIN28;    c-MYC;   

Others  :  1206643
DOI  :  10.1186/1477-7819-9-121

 received in 2011-07-27, accepted in 2011-10-07,  发布年份 2011

【 摘 要 】

Background

C-MYC, LIN28, OCT4, KLF4, NANOG and SOX2 are stem cell related factors. We detected whether these factors express in renal cell carcinoma (RCC) tissues to study their correlations with the clinical and pathological characteristics.

Methods

The expressions of c-MYC, LIN28, SOX2, KLF4, OCT4 and NANOG in 30 RCC patients and 5 non-RCC patients were detected with quantitative real-time reverse transcription-PCR (qRT-PCR). The data were analyzed with Wilcoxon signed rank sum test and x² test.

Results

In RCC group, c-MYC expression was significantly higher in RCC tissues compared with normal tissues (P < 0.05). The expression levels of OCT4, KLF4, NANOG and SOX2 were significantly lower in RCC tissues compared with normal tissues (P < 0.05). LIN28 expression level was not significant. No difference was observed when it comes to clinical and pathological characteristics such as gender, age, tumor size, cTNM classification and differentiation status (P > 0.05). Also the expression levels of all above factors were not significantly changed in non-RCC group (P > 0.05).

Conclusions

The present analysis strongly suggests that altered expression of several stem cell related factors may play different roles in RCC. C-MYC may function as an oncogene and OCT4, KLF4, NANOG and SOX2 as tumor suppressors.

【 授权许可】

   
2011 Liu et al; licensee BioMed Central Ltd.

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